DEACL - Departamento de Análises Clínicas

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Epitope mapping and protective immunity elicited by adenovirus expressing the Leishmania amastigote specific A2 antigen : correlation with IFN- and cytolytic activity by CD8+ T cells.
(2008) Resende, Daniela de Melo; Caetano, Bráulia Costa; Dutra, Míriam Santos; Abrantes, Christiane de Freitas; Verly, Rodrigo Moreira; Resende, Jarbas Magalhães; Veloso, Dorila Piló; Rezende, Simone Aparecida; Romero, Oscar Bruna; Fernandes, Ana Paula; Gazzinelli, Ricardo Tostes
A2was identified as an amastigote virulence factor of Leishmania (Leishmania) donovani and as a candidate antigen for vaccine development against visceral leishmaniasis. Here, predicted hydrophilic, class I and II MHC-binding synthetic peptides were used to define epitopes recognized by A2-specific antibodies, CD8+ T and CD4+ T cells, respectively. Immunization of BALB/c mice with adenovirus expressing A2 (AdA2) resulted in lowantibody response, contrasting with high levels of IFN-_ producing CD4+ T and CD8+ T cells specific for A2. Further, A2-specific CD8+ T cells from immunized mice were capable of lysing sensitized target cells in vivo. Finally, we demonstrated an association of A2-specific T cell responses and reduced parasitism in both liver and spleen from mice immunized with AdA2 and challenged with L. (L.) chagasi.