Browsing by Author "Barros, Camila Helena"

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    Atividade antinociceptiva, antiartrite gotosa e antioxidante de Lychnophora pinaster.
    (2019) Barros, Camila Helena; Guimarães, Dênia Antunes Saúde; Guimarães, Dênia Antunes Saúde; Costa, Luciana Souza Guzzo; Guimarães, Andrea Grabe
    A gota é uma enfermidade que acomete as articulações e tecidos subcutâneos devido à deposição de cristais de ácido úrico nestes locais, seguido de inflamação, dor e disfunção do membro atingido. Os fármacos disponíveis atualmente para o tratamento da artrite gotosa são reduzidos, sua farmacoterapia não abrange todos os sintomas da gota em apenas um medicamento e apresentam uma série de efeitos adversos, justificando a necessidade da busca por novas opções terapêuticas. As Lychnophoras, conhecidas popularmente como “arnica”, são espécies utilizadas na medicina popular na forma de soluções hidroalcóolicas ou pomadas para o tratamento da dor, reumatismo e inflamação. Pesquisas anteriores realizadas na UFOP demonstraram os efeitos do extrato etanólico da Lychnophora pinaster (EEL) sobre a hiperuricemia, inflamação e nocicepção. O presente estudo avaliou os efeitos do EEL e de seus constituintes químicos principais sobre a artrite induzida por cristais de urato monossódico (MSU) na articulação fêmur-tibial de camundongos C57BL/6 e sobre o estresse oxidativo. Como resultado da atividade antinociceptiva, o EEL na maior dose avaliada, reduziu o limiar de nocicepção de maneira equivalente a indometacina, fármaco anti-inflamatório (padrão) do experimento. Provavelmente as substâncias presentes no EEL, ácido cinâmico, quercetina, lupeol e estigmasterol foram responsáveis pelo efeito antinociceptivo de EEL, sendo que o lupeol foi mais efetivo que a indometacina. Na determinação da atividade antioxidante, o EEL não apresentou efeito sobre a atividade das enzimas superóxido dismutase (SOD) e catalase (CAT) no tecido periarticular. Das substâncias avaliadas, apenas vitexina promoveu o aumento da atividade da CAT e o ácido E-licnofórorico estimulou o aumento tanto da atividade da SOD quanto da CAT. A redução da migração de neutrófilos para a cavidade periarticular da articulação fêmur tibial dos camundongos foi observada nos grupos tratados com diferentes doses do EEL e nos grupos tratados com as substâncias presentes em L. pinaster, ácido cinâmico, rutina, quercetina, vitexina, lupeol, estigmasterol, ácido cafeico e ácido clorogênico. Neste trabalho o efeito anti-inflamatório do EEL observado, ocorreu pela inibição da migração de neutrófilos e pela redução das concentrações das citocinas próinflamatórias, IL-1β e TNF-α, na região inflamadada. Todas as substâncias detectadas no EEL contribuíram para os efeitos do extrato sobre a artrite. O ácido cinâmico, a rutina, ácido E-licnofórico e o estigmasterol demonstraram sua capacidade de inibição da IL-1β. Já o ácido cafeico e a vitexina promoveram a diminuição tanto da citocina IL-1β, como do TNFα. Dessa forma, o extrato etanólico de Lychnophora pinaster e seus constituintes principais se mostraram promissores para o tratamento da dor e inflamação decorrentes da artrite gotosa.
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    Coffea arabica extracts and their chemical constituents in a murine model of gouty arthritis : how they modulate pain and inflammation.
    (2022) Matosinhos, Rafaela Cunha; Bezerra, Juliana Pantaleão; Barros, Camila Helena; Bernardes, Ana Catharina Fernandes Pereira Ferreira; Araújo, Marcela Carolina de Paula Michel; Coelho, Grazielle Brandão; Soares, Rodrigo Dian de Oliveira Aguiar; Sachs, Daniela; Guimarães, Dênia Antunes Saúde
    Ethnopharmacological relevance: Coffea arabica is commonly known for its cardiotonic and neurotonic activities, but in some places’ folk medicine, like in Arabia and Africa, C. arabica is used to treat headache, migraine, the flu, anemia, oedema, asthenia, asthma, inflammation and wounds. Aims of the study: The aims were to evaluate if the aqueous extracts of Coffea arabica, prepared from beans with different degrees of roasting, and their main chemical constituents could exert an in vivo anti-gouty effect. Materials and methods: Coffea extracts were obtained from the beans of not roasted, light, medium and dark roasted coffee and from decaffeinated and traditional coffees and were prepared with water at 25◦C and at 98◦C. C57BL/6 mice were induced to gout by an injection of monosodium urate crystals and treated with coffee extracts at doses of 25, 75 and 225 mg/kg and their chemical constituents at a dose of 10 mg/kg. The antinociceptive and anti-inflammatory effects were evaluated. Results: Treatments with Coffea extracts prepared with water at 98◦C were more effective to exert antinociceptive and anti-inflammatory activities than the ones prepared with water at 25◦C. Caffeic and chlorogenic acids reduced hypernociception in animals when compared with negative control group (7.79 and 5.69 vs 18.53; P < 0.05 and P < 0.001, respectively), inhibited neutrophil migration (1.59 × 104 and 0.38 × 104 vs 9.47 × 104; P < 0.0001 both) and decreased pro-inflammatory cytokines concentration (IL-1β, IL-6 and TNF-α). Conclusions: We have demonstrated that our treatments attenuated gout, and this effect could be attributed to a reducement in hypernociception, neutrophil migration and cytokines concentration. These results suggest coffee as a potential candidate for studies in acute gout therapy.
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    Effects of Pimenta pseudocaryophyllus extracts on gout : anti-inflammatory activity and anti-hyperuricemic effect through xantine oxidase and uricosuric action.
    (2016) Ferrari, Fernanda Cristina; Lima, Rita de Cássia Lemos; Ferraz Filha, Zilma Schimith; Barros, Camila Helena; Araújo, Marcela Carolina de Paula Michel; Guimarães, Dênia Antunes Saúde
    Ethnopharmacological relevance: Leave sin fusion of Pimenta pseudocaryophyllus (Gomes) Landrum is used in Brazilian folk medicine to treat the predisposition to arthritical and gouty affections of the joints, fever and other diseases. A refreshing drink prepared with the specie is al soused due to its diuretic, sedative and aphrodisiac actions. Aim of the study: The study was under taken to investigate the mechanisms of anti-hyperuricemic effect and anti-inflammatory activity of P. pseudocaryophyllus extracts. Materials and methods: Anti-hyperuricemic effect was investigated using xanthine oxidase assay and uricosuric studies with rats in which hyperuricemia was induced by potassium oxonate and uric acid. Anti-inflammatory activity was investigate don MSU crystal-induced paw edema model. Ethyl acetate extracts of the leaves (EAL) and branches (EAB), ethanolic extracts of leaves (EEL) and branches (EEB) and aqueous extracts of leaves (AL) and branches (AB) were evaluated. Results: The extracts of P. pseudocaryophyllus evaluated showed expressive results regarding the in-hibition of xanthine oxidase enzyme in vitro and they were also able to reduce serum uric acid levels in hyperuricemicrats. The investigation of the mechanism of action, it was found that EAL, EAB, EEB, AB (125and250mg/kg) and AL (250mg/kg) promoted an increase on the urinary excretion of uric acid and EEL, EEB, AB (125and250mg/kg) and EAB (250mg/kg) were capable to inhibit liver xanthine oxidase. Treatments with EEL (125and250mg/kg) and EEB (250mg/kg) were able to reduce edema at 48thh. EAL and EAB (125and250mg/kg) showed significant anti-inflammatory activity on monosodium urate crystal-induced paw edema model at all evaluated times. Conclusions: The specie P. pseudocaryophyllus showed remark able anti-hyperuricemic effects through uricosuric effects and inhibition of xanthine oxidase and therefore can be considered as a promise in the treatment of diseases related to hyperuricemia. Moreover, ethyl acetate extracts had significant anti- inflammatoryactivity.
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    Lychnophora pinaster’s effects on inflammation and pain in acute gout.
    (2021) Barros, Camila Helena; Matosinhos, Rafaela Cunha; Bernardes, Ana Catharina Fernandes Pereira Ferreira; Araújo, Marcela Carolina de Paula Michel; Bezerra, Juliana Pantaleão; Sachs, Daniela; Soares, Rodrigo Dian de Oliveira Aguiar; Guimarães, Dênia Antunes Saúde
    Ethnopharmacological relevance: Ethanolic extract of aerial parts from Lychnophora pinaster Mart. are used in traditional Brazilian medicine for treating pain, rheumatism and inflammation. Aim of the study: Drugs for the treatment of gout present severe adverse effects, justifying the need to search for new therapeutic options. The aim of the present study was to evaluate the effects of the ethanolic extract of L. pinaster and its main constituents in arthritis induced in mice by the injection of monosodium urate (MSU) crystals. Materials and methods: Antinociceptive effect was investigated using an electronic pressure-meter nociception paw test in C57BL/6 mice. Anti-gouty arthritis was investigated in mice induced with gout by the injection of MSU crystals into their femur-tibial tissue. Ethanolic extract of the aerial parts of Lychnophora pinaster and its main chemical constituents were evaluated as treatment. Results: The ethanolic extract and their main chemical constituents inhibited neutrophil migration, reduced IL-1β and TNF-α concentrations in the inflamed tissue and showed antinociceptive activity. Conclusions: Gouty arthritis effects of the ethanolic extract can be attached to a synergistic effect of terpenes, flavonoids and phenolic acids present in the extract. Results obtained support the use of this extract and its main chemical constituents in the treatment of gout, inflammation, and pain.
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    Sesquiterpene lactones from Lychnophora species : antinociceptive, anti-inflammatory, and antioxidant pathways to treat acute gout.
    (2021) Bernardes, Ana Catharina Fernandes Pereira Ferreira; Matosinhos, Rafaela Cunha; Araújo, Marcela Carolina de Paula Michel; Barros, Camila Helena; Soares, Rodrigo Dian de Oliveira Aguiar; Costa, Daniela Caldeira; Sachs, Daniela; Guimarães, Dênia Antunes Saúde
    Ethnopharmacological relevance: Lychnophora trichocarpha and Lychnophora passerina are species used in folk medicine to treat inflammation, pain, and rheumatism. Previous studies have demonstrated the antiinflammatory effect of ethanol extracts of these species and identified that sesquiterpene lactones contribute to this activity. Aim of the study: Gout is an acute inflammatory arthritis caused by the deposition of monosodium urate (MSU) crystals in joints. Inflammation in joints induces oxidative stress in defense cells, releasing pro-inflammatory mediators. This study has three objectives: (1) to demonstrate the effects of sesquiterpene lactones lychnopholide and eremantholide C isolated from L. trichocarpha and goyazensolide isolated from L. passerina on arthritis induced by MSU crystals in C57BL6 mice; (2) to determine whether or not these compounds can inhibit the migration of neutrophils and the release of TNF-α and IL-1β cytokines in the inflammation region; and (3) to evaluate the effects of sesquiterpene lactones on the activities of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) in the cartilage of C57BL/6 mice with gouty arthritis. Materials and methods: The anti-inflammatory, antinociceptive, and antioxidant activities of sesquiterpene lactones in C57BL/6 mice with MSU crystal-induced arthritis were evaluated. In our experimental model, the mice were injected with MSU crystals in the tibiofemoral joint to induce arthritis and then treated with indomethacin, vitamin C, and sesquiterpene lactones. Nociception was evaluated before and after inflammation induction and treatments, neutrophil migration, IL-1β and TNF-α concentrations, and SOD and CAT activities. Results: Sesquiterpene lactones exerted an anti-inflammatory effect by inhibiting neutrophil migration and TNF-α production. These compounds also demonstrated antinociceptive and antioxidant activities. Conclusion: Lychnopholide, eremantholide C, and goyazensolide improved the inflammation induced by MSU crystals by inhibiting the migration of neutrophils to the inflamed area and by blocking the release of the proinflammatory cytokine TNF-α. In addition, sesquiterpene lactones reduced oxidative stress by activating SOD and CAT. These results suggest that sesquiterpene lactones have anti-gout activity through the inflammation, pain, and oxidative stress pathways.