Browsing by Author "Caligiorne, Rachel Basques"

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    Antioxidant activity, sensory analysis and acceptability of red fruit juice supplemented with Brazilian green propolis.
    (2022) Lopes, Géssica Aparecida; Fidelis, Priscila Cardoso; Almeida, Bruno de Moura; Almeida, Jane José; Ientz, Gislaine de Almeida Santana; Binda, Nancy Scardua; Teixeira, Arthur Ferrari; Vieira Filho, Sidney Augusto; Caligiorne, Rachel Basques; Guimarães, Dênia Antunes Saúde; Brumano, Maria Helena Nasser; Figueiredo, Sônia Maria de
    In this work were determining the total phenolic content, total flavonoids and antioxidant capacity in formulations of red fruit juice supplemented with different concentrations of aqueous extract of Brazilian green propolis. And, also was investigate the formulation sensory acceptance by volunteers’ consumers. The addition of propolis to red fruit juice increased the content of total phenolic compounds and flavonoids, enhanced the antioxidant activity of the final product as assessed by radical scavenging DPPH and ABTS and FRAP methods, and no negative effect on consumer taste. All red fruit juice formulations containing propolis presented significant results through overall acceptability test and were approved in relation to purchase intent by the volunteer consumers. Formulation F3 (6.1 mg.mL-1) showed the best results. The antioxidant activity increment was proportional to the concentration of aqueous green propolis extracts added to the juice. The positive purchase intention reached up 64.75% of participants’ acceptance. A new, sugar-free drink with pleasurable sensory and functional properties, combined with health benefits, has been obtained.
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    Canine visceral leishmaniasis : detection of Leishmania spp. genome in peripheral blood of seropositive dogs by real-time polymerase chain reaction (rt-PCR).
    (2019) Monteiro, Fabricio Moreira; Machado, Amanda Sanchez; Silva, Fabiana Rocha da; Assunção, Cláudia Barbosa; Melo, Cidiane Graciele da Silva; Costa, Lourena Emanuele; Portela, Aquila Serbate Borges; Coelho, Eduardo Antônio Ferraz; Figueiredo, Sônia Maria de; Caligiorne, Rachel Basques
    Visceral leishmaniasis (VL) is a zoonosis caused by the protozoa of the genus Leishmania. Among the species, L. infantum and/or L. infantum (chagasi) are the most important species affecting the Americas. Domestic dogs are the main reservoir of the parasite and participate effectively in the parasite' transmission cycle. The Canine Visceral Leishmaniasis Control Program (PCLV) adopted in Brazil present as strategies the vector control, health education and serological diagnosis of CVL in dogs followed by culling of the seropositive ones. The resolution to eliminate seropositive dogs by euthanasia, when necessary, are the most controversial and least accepted by society. The diagnostic methods for canine visceral leishmaniasis, currently indicated and approved in Brazil by the Ministry of Health from Brazil are the Dual Path Platform (DPP)® as a screening test and the Enzyme immunoassay test (ELISA®). This study aimed to verify the presence of Leishmania spp. DNA in peripheral blood samples of dogs presenting positive serological results byDPP® and ELISA® tests,throughreal-time polymerase chain reaction (rt-PCR), using the pair of primers 150–152 already described. For this purpose, were collected blood samples from 185 seropositive dogs among them, 41 (22%) exhibited some clinical signal of disease, whereas 144 (78%) was asymptomatic. The animals were also analyzed according to gender, race and hair size. According to the results of rt-PCR, it was observed that among the185 seropositive dogs analyzed, only 132 (71%) presented positive results for CVL and 53 (29%) presented negative results. From this, 41/41 symptomatic dogs were positive (100%), while among the asymptomatic dogs, 91/144 were positive (63, 2%) and 53/144 were negative (36, 8%). Concerning the hair size of seropositive dogs, we found that 41 (22%) had long hair, while 144 (78%) had short hair. No statistical significance occurred between the results of rt-PCR, ELISA and DPP tests and the profile of the animals (gender, size of the dogs and hair size), probably due to the small number of samples and the sampling differences of each profile. But statistical significance occurred between the results of rt-PCR and the clinical evaluation, since the rt-PCR was positive in all symptomatic dogs. Thus, through these results, we reached at the following question, which may contribute to an important current debate: the dogs presenting CVL seropositive diagnosis confirmed by tests distributed by the Ministry of Health were in reality ill or were they seropositive by living in an endemic area of the disease? Would these asymptomatic seropositive dogs spread the disease to the inhabitants even presenting a low parasite charge circulating in the blood.
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    Correlations among neck circumference and anthropometric indicators to estimate body adiposity in people living with HIV.
    (2021) Oliveira, Natália Alves; Guimarães, Nathalia Sernizon; Silva, Samantha Luiza Mazon e; Messias, Anny Caroline; Lopes, Gabriela Fonseca; Nascimento Júnior, Israel Borges do; Vieira Filho, Sidney Augusto; Caligiorne, Rachel Basques; Figueiredo, Sônia Maria de
    Neck circumference (NC) and anthropometric data of people living with HIV (PLWH) are correlated. Methods: Socioeconomic, NC, body mass index (BMI), tricipital skinfold thickness (TSF), mid-arm circumference (MAC), mid-arm muscle circumference (MAMC), waist-hip ratio (WHR), waist-to-height ratio (WHtR), waist circumference (WC), and hip circumference (HC) data of 72 PLWH were correlated. Results: Higher adiposity was observed in NC (40.3% [n=29]) and WC (31.9% [n=23]). Correlations between NC/BMI, NC/WC, NC/HC, NC/MAC, NC/MAMC, and NC/WHtR were significant. Increased NC (40.3%[n=29]) and WC (31.9 [n=23]) were associated with higher cardiometabolic risk. Conclusions: NC correlations are adequate for estimating cardiometabolic risk.
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    Oficinas culinárias aumentam a ingestão de fibras e melhoram o estado emocional e a qualidade de vida de pacientes diabéticos?
    (2020) Lopes, Géssica Aparecida; Guimarães, Nathalia Sernizon; Santos, Vanessa Fortes da Silva; Carvalho, Flávia Garcia; Souza, Anelise Andrade de; Vieira Filho, Sidney Augusto; Binda, Nancy Scardua; Caligiorne, Rachel Basques; Figueiredo, Sônia Maria de
    Introdução: Diabetes mellitus é uma doença metabólica de evolução crônica que pode resultar em complicações clínicas e prejuízos multidimensionais no estado emocional e na qualidade de vida dos pacientes. Objetivo: Comparar a ingestão de fibras dietéticas, o estado emocional e a qualidade de vida de diabéticos participantes e não participantes de oficinas culinárias. Métodos: Estudo observacional descritivo realizado em Ambulatório pertencente a uma universidade pública da Região dos Inconfidentes, Minas Gerais, com pacientes com diabetes mellitus do tipo 2, de ambos os sexos. As avaliações da ingestão dietética de fibras, estado emocional e qualidade de vida foram realizadas através da aplicação do recordatório de 24 horas, escala B-PAID e WHOQOL-BREF, respectivamente. Dados foram analisados pelo software Stata®, versão 13.0. Resultados: Ao total, 22 diabéticos tipo 2 participaram deste estudo, alocados em dois grupos: grupo 1 (G1) participantes de oficinas culinárias (n=11) e grupo 2 (G2) não participantes das oficinas culinárias (n=11). A média do consumo de fibras dietéticas foi de 24,6 g para o G1 e 19,7g para o G2 (p<0.01). Quanto ao escore de avaliação do estado emocional, observou-se que os participantes de oficinas culinárias obtiveram 11,7 pontos e não participantes 30,7 pontos (p<0.01). Participantes de oficinas culinárias relataram qualidade de vida “boa” em 81,8% dos casos em detrimento de 18,2% dos não participantes das oficinas culinárias (p=0.013). Conclusão: Os diabéticos participantes das oficinas culinárias relataram maior ingestão de fibras, melhores estado emocional e qualidade de vida de pacientes diabéticos.
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    Physicochemical characteristics of Brazilian green propolis evaluated during a six-year period.
    (2017) Figueiredo, Sônia Maria de; Binda, Nancy Scardua; Vieira Filho, Sidney Augusto; Almeida, Bruno de Moura; Abreu, Sheila Rago Lemos; Paulino, Niraldo; Pastore, Gláucia Maria; Sato, Hélia Harumi; Theodoropoulos, Viviane Cristina Toreti; Tapia, Eulália Vargas; Park, Yong Kun; Caligiorne, Rachel Basques
    Background: Propolis has been used as a natural health product mainly due to the presence of polyphenols, flavonoids, phenolic aldehydes, amino acids, vitamins and others bioactive constituents. To this natural substance are attributed different biological and pharmacological properties which are influenced by its chemical composition and organoleptic properties. The aim of this work was to evaluate the physicochemical properties and parameters of green propolis collected during a period of six years (2008-2013) in the state of Minas Gerais, located at the southeastern region of Brazil. Methods: The methodology were in accordance with Brazilian legislation on the identity and quality standards of propolis. The evaluated parameters of hydroalcoholic from green propolis were total flavonoids, antioxidant activity - DPPH method, oxidation index, wax content, humidity and insoluble impurities. Results: Propolis samples collected in different seasons during the years 2008 to 2013 presented mean values of total flavonoids (3.4 ± 0.11 mg/g), antioxidant activity DPPH (4.76 ± 0.16 μg/mL), oxidation index (3, 4 ± 0.33 seconds) and wax (15.14 ± 0.78% m/m), which are in accordance with Brazilian legislation. Conclusion: Green propolis did not show abrupt seasonal changes during the six years of investigation, and may be considered as an adequate functional ingredient.
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    Própolis verde e vermelha : atividade antifúngica.
    (2018) Freitas, Márcia Christina Dornelas de; Figueiredo, Sônia Maria de; Johann, Susana; Caligiorne, Rachel Basques; Silva, Marcelo Eustáquio; Figueiredo, Sônia Maria de
    A candidíase é uma infecção fúngica causada por espécies do gênero Candida, potencialmente patogênicas, que vem acometendo muitos indivíduos devido ao crescente número de imunossuprimidos e à resistência aos antifúngicos comumente utilizados. Este estudo foi desenvolvido no intuito de gerar conhecimento que possa auxiliar na busca de alternativas para o tratamento de infecções fúngicas. O principal objetivo foi avaliar in vitro e in vivo a atividade de extratos de própolis verde e própolis vermelha contra espécies do gênero Candida spp. No teste in vitro, utilizouse cepas padronizadas American Type Culture Collection (ATCC): C. albicans ATCC SC5314 e ATCC 18804, C. tropicalis ATCC 750, C. krusei ATCC 20298, C. glabrata ATCC 2001 e C. parapsilosis ATCC 22019, e 10 isolados clínicos pertencentes à Coleção de microorganismos e Células da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil. Foi determinada a Concentração Inibitória Mínima (CIM) de extrato de própolis verde e vermelha e cápsulas comerciais de própolis verde. Verificou-se a ação sinérgica de quatro extratos de própolis com fluconazol e com anfotericina B. do crescimento C. albicans pelas própolis na adesão de células bucais. Já a ação das própolis in vivo foi avaliada no ensaio de sobrevivência com as larvas de Tenebrio molitor. Nos resultados in vitro, observou-se que todos os extratos foram capazes de inibir ao menos uma das cepas testadas, se destacando os extratos de própolis vermelho a 25% e de própolis verde a 100% na inibição do crescimento de todas as Candidas padrões testadas, suas concentrações variaram de 125µg/mL a 1000µg/mL e 500µg/mL a 2000µg/mL, respectivamente. Foi também comprovado in vitro que as própolis possuem atividade sinérgica tanto com anfotericina B como com fluconazol. Nos resultados dos Testes de Adesão de Células Epiteliais Bucais, verificou-se inibição dos extratos GrProp50, GrProp28, GrProp100, RedProp25 (42,60,62 e 80%respectivamente, sendo esses resultados estatisticamente similar à inibição da anfotericina B (p>0,05). Já em relação à sobrevivência com as própolis testadas demonstraram ser eficazes no tratamento de larvas de Tenebrio molitor, aumentando sua sobrevida. Esses resultados mostraram que tanto a própolis verde quanto a vermelha possuem grandes chances de ser empregado em tratamento de candidíase, devido à ação antifúngica isolada ou sinérgica com outras drogas antifúngicas.
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    The anti-oxidant properties of isothiocyanates : a review.
    (2013) Figueiredo, Sônia Maria de; Vieira Filho, Sidney Augusto; Machado, José Augusto Nogueira; Caligiorne, Rachel Basques
    Cruciferous vegetables, such as broccoli and watercress, have been studied extensively aiming to evaluate their chemopreventive properties. Some of them have already been established using animal models. The ITCs induce Phase II enzymes related to detoxification processes of chemical carcinogens to prevent the start of carcinogenesis. They also exhibit antitumor activity at post-initiation phase, suggesting their additional role(s) in cancer prevention. Sulforaphane is the most extensively studied isothiocyanate, focused in its anti-tumoral activity and it is mainly found in great amounts in broccoli and other cruciferous. In a dose dependent manner, ITCs inhibit the cell viability of human cervical cancer cells, human pancreatic cancer cells, human hepatocellular carcinoma cells, human ovarian cancer cells, and have antiinflammatory properties in the treatment of human T-cell leukemia cells. This protective effect may be due to improved antioxidant status. Although the health effects of diet in humans are generally considered promising, there are definite challenges and limitations of the current data in better understanding of the molecular mechanisms responsible for this effect, together with the possible interactions between different dietary constituents. The survey of relevant patents on the use of isothiocyanates such as sulforaphane for cancer and cardiovascular diseases treatments is also included in this review.