Browsing by Author "Cosenza, Gustavo Pereira"

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Anti-helminthic activity of Momordica charantia L. against Fasciola hepatica eggs after twelve days of incubation in vitro.
    (2016) Pereira, Cíntia Aparecida de Jesus; Oliveira, Laura Lúcia dos Santos; Lucas, Aytube Coaglio Silva; Santos, Fernanda Stéfani Oliveira dos; Cezar, Rodolfo Silva Moreira; Mendes, Tiago Antônio de Oliveira; Oliveira, Fernando Luiz Pereira de; Cosenza, Gustavo Pereira; Lima, Walter dos Santos
    Fasciolosis, a parasitic disease caused by the trematode Fasciola hepatica underreported is expanding both in human and animal population, throughout the world. The constant use of synthetic drugs to treat this condition has led to the natural selection of resistant strains of the parasite. Hence, there is a growing focus on the potential anti-helminthic properties of medicinal plants and phytopharmaceuticals. The current study assessed the potential anti-fasciolicide action of Momordica charantia leaf extracts and fractions on the eggs of F. hepatica parasites. The lyophilized crude extract (CE) of M. charantia leaves and its sub-fractions, obtained from liquid–liquid partitioning with organic solvents, were analysed by High Performance Liquid Chromatography (HPLC), suspended in 1% DMSO and used in in vitro tests. Quadruplicates of 50 F. hepatica eggs were incubated at 23 ◦C with M. charantia leaf CE in different concentrations. After 12 days no larvae were formed in eggs incubated with CE concentrations above 12.5 mg/mL. Eggs incubated with CE sub-fractions at concentrations of 1000, 100, 10, 1, 0.1, 0.01 g/mL affected embryonic development, with n-butanol presenting the strongest inhibition of miracidia formation. In contrast, on the 12th day, 90% of the miracidia hatched in the control experiments using 0.03% DMSO whereas embryogenesis was completely abolished with any concentration of albendazole sulphoxide ABZ(SO). Chemical analysis of the CE and sub-fractions revealed a prominent presence of flavonoids. HPLC–MS confirmed Quercetin to be one of the main flavonoids present in the CE and the n-butanol subfraction. This is the first study to analyse the potential anti-fasciolicide action of M. charantia leaf CE and subfractions.
  • No Thumbnail Available
    Item
    Different source of commercial vegetable oils may regulate metabolic, inflammatory and redox status in healthy rats.
    (2020) Gomes, Sttefany Viana; Dias, Bruna Vidal; Pereira, Renata Rebeca; Lúcio, Karine de Pádua; Souza, Débora Maria Soares de; Silva, André Talvani Pedrosa da; Brandão, Geraldo Célio; Cosenza, Gustavo Pereira; Queiroz, Karina Barbosa de; Costa, Daniela Caldeira
    Our goal was to carry out a comparative study to evaluate the metabolic and inflammatory effects and the redox status of commercial vegetable oils supplementation [linseed (LO), coconut (VCO), and sunflower (SO)] in metabolically healthy rats. The results found in this study showed that the LO group decreased the HOMA-IR and hepatic cholesterol, and increased the serum levels of IL-6. Supplementation with VCO increased glucose and HOMA-IR, cholesterol concentration and serum triacylglycerol (TAG). In this group, there was also an increase in TBARS. In the SO group there was a decrease in serum concentrations of cholesterol and TAG and an increase in hepatic concentration of these lipids. In addition, in the SO group there was a decrease in hepatic and sérum concentrations of IL-6 and hepatic levels of TNF, as well as a decrease in the GSH/GSSG ratio, suggesting changes in glutathione metabolism and inflammatory mediators.