Browsing by Author "Gomes, Sttefany Viana"

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    Açaí (Euterpe oleracea Martius) promotes jejunal tissue regeneration by enhancing antioxidant response in 5-Fluorouracil-Induced mucositis.
    (2020) Magalhães, Talita Alves Faria Martins; Souza, Melina Oliveira de; Gomes, Sttefany Viana; Silva, Raiana Mendes e; Martins, Flaviano dos Santos; Freitas, Renata Nascimento de; Amaral, Joana Ferreira do
    Intestinal mucositis (IM) caused by antineoplastic chemotherapy is characterized by an important inflammatory process, which may compromise ongoing treatment. Our study aimed to investigate the effect of Ac¸aı (Euterpe oleracea Martius) on the antioxidant response in BALB/c mice pretreated with Ac¸aı pulp (200 g/kg) for 14 day. A group of animals receiving a single intraperitoneal injection of 5-FU (200 mg/kg) were euthanized on day three (D3) or seven (D7) after administration, the distal jejunum was isolated for the analyses of histology, superoxide dismutase (SOD) and catalase (CAT) enzyme activities, and concentration of total sulfhydryl groups (GSH). Seven days after induction, the intake of Ac¸aı by the IM group almost completely regenerated tissue histology. Notably, SOD activity decreased in the MUC þ Ac¸aı group (D3). CAT activity reduced on D3 and D7 in the IM groups and Ac¸aı treatment groups, respectively. No change was observed in the total GSH concentration at the tissue level. Our results demonstrated the protective effect of Ac¸aı pulp components on intestinal damage induced by 5-FU, as well as the ability to control the response to oxidative stress, in order to mobilize defense pathways and promote tissue repair.
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    Different source of commercial vegetable oils may regulate metabolic, inflammatory and redox status in healthy rats.
    (2020) Gomes, Sttefany Viana; Dias, Bruna Vidal; Pereira, Renata Rebeca; Lúcio, Karine de Pádua; Souza, Débora Maria Soares de; Silva, André Talvani Pedrosa da; Brandão, Geraldo Célio; Cosenza, Gustavo Pereira; Queiroz, Karina Barbosa de; Costa, Daniela Caldeira
    Our goal was to carry out a comparative study to evaluate the metabolic and inflammatory effects and the redox status of commercial vegetable oils supplementation [linseed (LO), coconut (VCO), and sunflower (SO)] in metabolically healthy rats. The results found in this study showed that the LO group decreased the HOMA-IR and hepatic cholesterol, and increased the serum levels of IL-6. Supplementation with VCO increased glucose and HOMA-IR, cholesterol concentration and serum triacylglycerol (TAG). In this group, there was also an increase in TBARS. In the SO group there was a decrease in serum concentrations of cholesterol and TAG and an increase in hepatic concentration of these lipids. In addition, in the SO group there was a decrease in hepatic and sérum concentrations of IL-6 and hepatic levels of TNF, as well as a decrease in the GSH/GSSG ratio, suggesting changes in glutathione metabolism and inflammatory mediators.
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    Efeito do consumo crônico de diferentes óleos vegetais sobre a modulação de mediadores metabólicos, do status redox e inflamatório em ratas saudáveis.
    (2018) Gomes, Sttefany Viana; Costa, Daniela Caldeira; Queiroz, Karina Barbosa de; Silva, Marcio Eustáquio; Magalhães, Cíntia Lopes de Brito; Alzamora, Andréia Carvalho; Queiroz, Karina Barbosa de; Costa, Daniela Caldeira
    O processo de transição nutricional observado em todo o mundo tem sido caracterizado por um aumento da ingestão de alimentos com elevada densidade calórica, ricos em carboidratos e/ou lipídeos, em especial gordura saturada, trans e ácido linoleico. O desequilíbrio na quantidade e no tipo de lipídeos consumidos na dieta pode ter efeitos negativos no organismo. Os óleos vegetais têm recebido atenção especial devido aos potenciais efeitos benéficos à saúde. Estes efeitos estão associados ao tipo de ácido graxo presente nestes óleos, sendo os ácidos graxos monoinsaturados e poli-insaturados associados à melhora no perfil lipídico, enquanto os ácidos graxos saturados apresentam efeitos negativos no organismo. Desta forma, o objetivo do presente estudo foi avaliar o efeito do consumo crônico de óleos vegetais com diferentes percentuais de ácidos graxos sobre mediadores metabólicos, do status redox e inflamatórios em ratas saudáveis. Para isto, foram utilizadas 44 ratas da linhagem Fisher (peso), separadas em cinco grupos, a saber: (i) grupo controle (C), o qual recebeu apenas água, (ii) grupo azeite de oliva (AO), (iii) grupo óleo de linhaça (OL), (iv) grupo óleo de coco (OC) e (v) grupo óleo de girassol (OG). Todos os tratamentos foram administrados por gavagem (1mL/ 250g peso) e o delineamento experimental teve duração de 90 dias. Após este período, as ratas foram eutanasiadas e amostras de sangue, fígado e tecido adiposo foram coletadas e armazenadas para análises posteriores. O consumo crônico de AO aumentou o tecido adiposo branco e a concentração sérica de IL-6, enquanto reduziu o HDL sérico. Os animais do grupo OL apresentaram redução do índice HOMA, aumento nas concentrações de triglicérides séricos, ALT e IL-6, além de aumentarem TNF hepático. A ingestão crônica de OC foi responsável por alterações de parâmetros glicometabólicos e modificações no metabolismo lipídico sérico e hepático, além de aumentar o dano oxidativo hepático através do aumento de TBARS e ALT. Os animais do grupo OG apresentaram alteração no perfil lipídico sérico, aumento da lipogênese hepática, através do aumento da expressão gênica do fator de transcrição Srebp1 e da enzima Acetil-Coa carboxilase (ACC). Além disso, o OG causou alterações nas concentrações séricas e hepáticas de citocinas inflamatórias, aumento de dano hepático e diminuição da razão GSH/GSSG. Estes resultados sugerem que os diferentes ácidos graxos presentes nos óleos vegetais interferem na homeostase do organismo, podendo contribuir para a patogênese de diversas doenças em ratas saudáveis. Desta forma, mais estudos precisam ser feitos a respeito da quantidade e qualidade dos lipideos dietéticos a fim de se promover recomendações para um consumo consciente e adequado dos mesmos.
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    EPA/DHA and linseed oil have different effects on liver and adipose tissue in rats fed with a high-fat diet.
    (2022) Dias, Bruna Vidal; Gomes, Sttefany Viana; Castro, Maria Laura da Cruz; Carvalho, Luana Cristina Faria; Breguez, Gustavo Silveira; Souza, Débora Maria Soares de; Ramos, Camila de Oliveira; Sant’Ana, Marcella Ramos; Nakandakari, Susana Castelo Branco Ramos; Araujo, Carolina Morais; Guimaraes, Andrea Grabe; Silva, André Talvani Pedrosa da; Carneiro, Cláudia Martins; Cintra, Dennys Esper Corrêa; Costa, Daniela Caldeira
    The incidence of cardiovascular diseases and metabolic disorders has increased worldwide. Clinical and exper- imental research has shown that the consumption of ω-3 FAs can be beneficial to metabolism in several ways, as they can act on metabolic pathways. Our objective was to evaluate the effect of treatment with linseed oil, a vegetable oil rich in alpha-linolenic acid, and EPA and DHA in different proportions (3:1 EPA:DHA, and 1:3 EPA: DHA), on the metabolic disorders induced by a high-fat diet (20 % lipids) in rats for 2 weeks, after 18 weeks of consumption of a high-fat diet. In 18 weeks, the high-fat diet increased blood glucose, systolic blood pressure, triglyceride concentration in the liver and adipose tissue, and impaired insulin sensibility without interfering in the weight of the animals. All treatments were effective in reducing the deposition of hepatic type III collagen, the proportion of ω-6/ω-3 in the liver and WAT (white adipose tissue), the proportion of area/number of adi- pocytes, and the gene expression of the ACC, FAS, and CPT1 enzymes. In addition, treatment with EPA and DHA reduced blood glucose, serum TNF-α concentration, amount of liver fat, degree of microsteatosis and type I collagen deposition in the liver, deposition of type I and III collagen in TA, gene expression of the transcription factor SREBP-1c, and increased hepatic binucleation. EPA in major proportion was more effective in reducing the area of adipocytes, hepatic triglyceride concentration, PPAR-α expression, and WAT fat weight. DHA in a major proportion reduced the concentration of MCP1 in WAT. LO treatment did not have any isolated effects. We concluded that EPA and DHA were more effective in treating metabolic damage than treatment with LO, leading to a more favorable metabolic profile.