Browsing by Author "Lemos, Elenice Moreira"

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    Canine visceral leishmaniasis : performance of a rapid diagnostic test (Kalazar Detect TM ) in dogs with and without signs of the disease.
    (2008) Lemos, Elenice Moreira; Laurenti, Marcia Dalastra; Moreira, Márcio Antônio Batistela; Reis, Alexandre Barbosa; Giunchetti, Rodolfo Cordeiro; Raychaudhuri, Syamal; Dietze, Reynaldo
    Current visceral leishmaniasis (VL) control programs in Brazil include the infected dog elimination but, despite this strategy, the incidence of human VL is still increasing. One of the reasons is the long delay between sample collection, analysis, control implementation and the low sensitivity of diagnostic tests. Due to the high prevalence of asymptomatic dogs, the diagnosis of these animals is important considering their vector infection capacity. Hence, a rapid and accurate diagnosis of canine visceral leishmaniasis is essential for an efficient surveillance program. In this study we evaluated the performance of rK39 antigen in an immunochromatographic format to detect symptomatic and asymptomatic Leishmania chagasi infection in dogs and compared the results with those using a crude antigen ELISA. The sensitivity of rK39 dipstick and ELISA were 83% vs. 95%, respectively, while the specificity was both 100%. Our results also demonstrated that the dipstick test was able to detect infected dogs presenting different clinical forms.
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    First description of autochthonous canine visceral leishmaniasis in the metropolitan region of Vitória, State of Espírito Santo, Brazil.
    (2012) Tonini, Marco André Loureiro; Lemos, Elenice Moreira; Reis, Alexandre Barbosa; Vital, Wendel Coura; Dias, Edelberto Santos; Dietze, Reynaldo
    Introduction: We investigated autochthonous canine visceral leishmaniasis (CVL) in the metropolitan region of Vitória (MRV), an area in which a human case was previously reported. Methods: Serological, parasitological, and molecular tests were performed in 201 dogs. Results: Twenty-six (13%) and 12 (6%) dogs were identified as positive using in-house enzyme-linked immunosorbent assay (ELISA) and rK39 tests, respectively. Two dogs had a positive culture for Leishmania chagasi, and 4 were polymerase chain reaction (PCR)-positive for Leishmania spp. One positive dog belonged to the aforementioned patient. Conclusions: Although the responsible vector was not found, our results provide evidence of autochthonous CVL in the MRV, a non-endemic area for VL.
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    Laboratorial algorithm for serological diagnosis of visceral leishmaniasis using rK39-ICT, DAT-LPC and FC-Simplex IgG1.
    (2020) Vale, Isabela Natália Pascoal Campos do; Saliba, Juliana Wilke; Fonseca, Giuliana Schmidt França; Pascoal, Vanessa Peruhype Magalhães; Araújo, Fernanda Fortes de; Xavier, Marcelo Antônio Pascoal; Carvalho, Andréa Teixeira de; Campos, Fernanda Magalhães Freire; Andrade, Mariléia Chaves; Lula, Jamille Fernandes; Reis, Alexandre Barbosa; Lemos, Elenice Moreira; Carvalho, Sílvio Fernando Guimarães de; Oliveira, Edward; Martins Filho, Olindo Assis
    The performance of distinct serological tests (rK39-ICT, IFAT, DAT-LPC, FC-Simplex IgG1) was assessed and a laboratorial algorithm was proposed for accurate diagnosis of VL. DAT-LPC and FC-Simplex IgG1 showed outstanding accuracy (AUC = 0.93) to identify VL patients. The use of a sequential serological algorithm (rK39-ICT screening followed by DAT-LPC or FC-Simplex IgG1) improved the global accuracy for VL (97.2%) diagnosis. An alternative approach for diagnosis of VL has been also assessed for interchangeable use of serum/whole blood lysate samples in DAT-LPC and FC-Simplex IgG1. Our data showed an outstanding agreement for the results obtained with whole blood lysate samples as compared to serum samples (DAT-LPC =100%; FC-Simplex IgG1 = 99%). Together, these findings provide insights to improve the current overall accuracy of VL diagnosis and present innovative laboratorial tests and alternative samples from use in public health services.
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    Leishmania (Viannia) braziliensis : immunoblotting analysis for the detection of IgG subclasses in the diagnosis of symptomatic and asymptomatic dogs.
    (2010) Zanini, Marcos Santos; Viana, Kelvinson Fernandes; Reis, Alexandre Barbosa; Campos, Diefrey Ribeiro; Mussi, Jamili Maria Suhet; Zanini, Surama; Lemos, Elenice Moreira
    During a seroepidemiological survey 2004–2006 from areas in Brazil endemic for American cutaneous leishmaniasis (ACL), serum samples from 10 dogs with ulcerated cutaneous lesions (S-ACL) and 52 asymptomatic dogs (AS-ACL) of unknown age and breed living in areas endemic for ACL were monitored for 1 year for ulcerated cutaneous lesions and immunoblotting using peroxidase-conjugated secondary anti-IgG, anti-IgG1 and anti-IgG2 dog antibodies. We reported that antibodies against Leishmania (Viannia) braziliensis in the sera of 22/52 dogs with asymptomatic disease showed intense reactivity to pep tides larger than 66 kDa. We believe that dogs harboring subclinical amastigotes show an immunoblotting profile similar to that of symptomatic animals because a dog with self-healing presented antigens greater than 66 kDa. Such patterns can be exploited for diagnostic and epidemiological research for leishmaniasis.