Browsing by Author "Lula, Jamille Fernandes"

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    Laboratorial algorithm for serological diagnosis of visceral leishmaniasis using rK39-ICT, DAT-LPC and FC-Simplex IgG1.
    (2020) Vale, Isabela Natália Pascoal Campos do; Saliba, Juliana Wilke; Fonseca, Giuliana Schmidt França; Pascoal, Vanessa Peruhype Magalhães; Araújo, Fernanda Fortes de; Xavier, Marcelo Antônio Pascoal; Carvalho, Andréa Teixeira de; Campos, Fernanda Magalhães Freire; Andrade, Mariléia Chaves; Lula, Jamille Fernandes; Reis, Alexandre Barbosa; Lemos, Elenice Moreira; Carvalho, Sílvio Fernando Guimarães de; Oliveira, Edward; Martins Filho, Olindo Assis
    The performance of distinct serological tests (rK39-ICT, IFAT, DAT-LPC, FC-Simplex IgG1) was assessed and a laboratorial algorithm was proposed for accurate diagnosis of VL. DAT-LPC and FC-Simplex IgG1 showed outstanding accuracy (AUC = 0.93) to identify VL patients. The use of a sequential serological algorithm (rK39-ICT screening followed by DAT-LPC or FC-Simplex IgG1) improved the global accuracy for VL (97.2%) diagnosis. An alternative approach for diagnosis of VL has been also assessed for interchangeable use of serum/whole blood lysate samples in DAT-LPC and FC-Simplex IgG1. Our data showed an outstanding agreement for the results obtained with whole blood lysate samples as compared to serum samples (DAT-LPC =100%; FC-Simplex IgG1 = 99%). Together, these findings provide insights to improve the current overall accuracy of VL diagnosis and present innovative laboratorial tests and alternative samples from use in public health services.
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    Níveis plasmáticos de TNF-alfa, TRAIL e FasLigante/CD95L correlacionados com a disfunção ventricular em pacientes com cardiopatia chagásica crônica Ouro Preto 2008.
    (Programa de Pós-Graduação em Ciências Biológicas. Núcleo de Pesquisas em Ciências Biológicas, Pró-Reitoria de Pesquisa e Pós Graduação, Universidade Federal de Ouro Preto., 2008) Lula, Jamille Fernandes; Silva, André Talvani Pedrosa da
    Apoptose de cardiomiócitos tem sido reportado estar envolvido na patogênese da cardiomiopatia chagásica crônica (CCC). Para determinar se a maquinaria apoptótica está correlacionada aos distúrbios arrítmicos ventriculares e funcionais na CCC, foi examinado os ligantes da superfamília do TNF (TNF-alfa, Fasligante/CD95L e TRAIL), quanto a sua capacidade de ativar o receptor da via de morte, no plasma de indivíduos chagásicos com insuficiência cardíaca tanto branda e grave quanto de indivíduos nãoinfectados correlacionando clinicamente (ecocardiografia e eletrocardiografia) em relação aos dados bioquímicos. A concentração de TNF-alfa e TRAIL foi claramente aumentada somente nos indivíduos com a forma grave de CCC enquanto Fas ligante/CD95L foi detectada em todos os indivíduos com doença de Chagas. Todos os ligantes de TNF apresentaram baixas concentrações no plasma de indivíduos de voluntários não-infectados pelo parasito Trypanosoma cruzi. Uma boa correlação foi encontrada entre TNF-alpha (r=0.85 e r=0.68) ou TRAIL (r=0.68 e r=0.60) e os parâmetros funcionais ventriculares como fração de ejeção ventricular esquerda (LVEF) e diâmetro diastólico ventricular esquerdo (LVDD), respectivamente. Apesar da sensibilidade destes, Fas ligante/CD95L apresentou uma fraca correlação (r=0.50) para ambos LVEF e LVDD. Curiosamente, os ligantes da superfamília do TNF foram mais correlacionados somente com os parâmetros funcionais, mas não com eventos arrítmicos. Mais estudos são necessários ainda, mas juntos nossos dados sugerem uma participação de eventos apoptóticos em distúrbios funcionais do ventrículo esquerdo durante a CCC e uma intervenção precoce na via de apoptose poderá preservar alguns parâmetros funcionais cardíacos durante a doença de Chagas crônica.
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    Plasma concentrations of tumour necrosis factor-alpha, tumour necrosis factor-related apoptosis-inducing ligand, and FasLigand/CD95L in patients with Chagas cardiomyopathy correlate with left ventricular dysfunction.
    (2009) Lula, Jamille Fernandes; Rocha, Manoel Otávio da Costa; Nunes, Maria do Carmo Pereira; Ribeiro, Antônio Luiz Pinho; Teixeira, Mauro Martins; Bahia, Maria Terezinha; Silva, André Talvani Pedrosa da
    Cardiomyocyte apoptosis is reported to be involved in the pathogenesis of human chronic Chagas cardiomyopathy (CCC). Members of the tumour necrosis factor (TNF) superfamily (TNF-a, FasLigand/CD95L, and TNF-related apoptosis-inducing ligand) are known to activate the death receptor pathway. We therefore investigated whether levels of TNF-a, FasLigand/CD95L, and TRAIL correlated with changes in heart function of patients with Chagas disease (n ¼ 31). Concentrations of TNF-a and TRAIL were clearly augmented in individuals with severe form CCC (n ¼ 16). Levels of FasLigand/CD95L were greater in chagasic patients than in non-infected individuals (n ¼ 15) but did not differentiate between clinical forms of Chagas disease. There was a good correlation between TNF-a (r ¼ 0.85 and r ¼ 0.68, P , 0.0001) or TRAIL (r ¼ 0.68 and r ¼ 0.60, P , 0.001) and left ventricular ejection fraction (LVEF) and left ventricular diastolic diameter (LVDD), respectively. In addition, TNF-a (r ¼ 0.57, P ¼ 0.0001), TRAIL (r ¼ 0.56, P ¼ 0.001), and FasLigand/CD95L (r ¼ 0.51, P ¼ 0.001) showed a good correlation with brain natriuretic peptide, a well-known parameter of ventricular dysfunction in CCC. There was a weak correlation between levels of FasLigand/CD95L (r ¼ 0.50, P , 0.004) and both LVEF and LVDD. There was no correlation between levels of TNF superfamily ligands and chronotropic incompetence, maximal heart rate, or number of ventricular premature beats in 24 h. Plasma levels of TNF superfamily ligands are elevated in patients with functional but not arrhythmogenic disturbances, and these death receptor ligands may be potential markers of ventricular dysfunction in CCC.