Browsing by Author "Moraes Filho, Manoel Odorico de"

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    Bioactive extracts and chemical constituents of two endophytic strains of Fusarium oxysporum.
    (2012) Nascimento, Andréa Mendes do; Conti, Raphael; Turatti, Izabel Cristina Casanova; Cavalcanti, Bruno Coêlho; Lotufo, Leticia Veras Costa; Pessoa, Cláudia; Moraes Filho, Manoel Odorico de; Manfrim, Viviane; Toledo, Juliano Simões de; Cruz, Angela Kaysel; Pupo, Mônica Tallarico
    Ethyl acetate extracts of cultures grown in liquid Czapek and on solid rice media of the fungal endophyte Fusarium oxysporum SS46 isolated from the medicinal plant Smallanthus sonchifolius (Poepp.) H. Rob., Asteraceae, exhibited considerable cytotoxic activity when tested in vitro against human cancer cells. Chromatographic separation yielded anhydrofusarubin (1) and beauvericin (2) that were identified based on their 1H and 13C NMR data. Compounds 1 and 2 showed the strongest cytotoxic activity against different cancer cell lines. Compound 2 also showed promising activity against Leishmania braziliensis. Hexanic extract of F. oxysporum SS50 grown on solid rice media also afforded a mixture of compounds that displayed cytotoxic activity against different cancer cell lines. Chemical analysis of the mixture of compounds, investigated by gas chromatography-mass spectrometry (GC-MS), showed that there was a predominance of methyl esters of fatty acids and alkanes.
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    In vitro activity evaluation of seven Brazilian Asteraceae against cancer cells and Leishmania amazonensis.
    (2019) Silva, Ana Cláudia Nogueira da; Nascimento, Renato Malveira Carreiro do; Rodrigues, Débora Caroline do Nascimento; Ferreira, Paulo Michel Pinheiro; Pessoa, Claudia do Ó; Lima, Daisy Jereissati Barbosa; Moraes Filho, Manoel Odorico de; Almeida, Raquel Martins de; Ferreira, Sebastião Rodrigo; Fujiwara, Ricardo Toshio; Nascimento, Andréa Mendes do
    Natural products are a very productive leading source of compounds for the development of medicines. The aim of this research was to investigate the in vitro antiproliferative and antileishmanial activities of 21 extracts from 7 Asteraceae species. Dried aerial parts of Asteraceae plant species were extracted using organic solvents in order of increasing polarities. Antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method, and nine extracts (43%) displayed moderate to high in vitro antiproliferative activity against human cancer cell lines HCT-116, OVCAR-8 and SF-295. Two crude extracts displaying antiproliferative activity were fractionated and yielded a fraction rich in lupeol acetate, a fraction rich in lupeol and 5,7,3′,4′-tetrahydroxyflavone-7-O-β-D-glucopyranoside. The antileishmanial activity was evaluated by amastigotes growth inhibition test in Leishmania amazonensis, and three extracts showed promising activity. Phytochemical screening of all plant extracts was also made for terpenoids, flavonoids, tannins, alkaloids and saponins.