Browsing by Author "Oliveira Neto, Ari Soares de"

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    Amphotericin B-loaded Eudragit RL100 nanoparticles coated with hyaluronic acid for the treatment of vulvovaginal candidiasis.
    (2020) Melo, Carolina Mirtes; Cardoso, Jéssica Ferreira; Perasoli, Fernanda Barçante; Oliveira Neto, Ari Soares de; Pinto, Luccas Moreira; Marques, Maria Betânia de Freitas; Mussel, Wagner da Nova; Magalhães, Juliana Teixeira de; Moura, Sandra Aparecida Lima de; Araújo, Marcelo Gonzaga de Freitas; Silva, Gisele Rodrigues da
    The treatment of vulvovaginal candidiasis (VVC) is based on oral and vaginal formulations which show limited effectiveness. In this study, amphotericin B-loaded Eudragit RL100 nanoparticles coated with hyaluronic acid (AMP EUD nanoparticles/HA) were developed to overcome the drawbacks of the conventional formulations. AMP EUD nanoparticles/HA were synthesized by nanoprecipitation, formulated by statistical experimental design, and characterized. AMP release from EUD nanoparticles/HA and its antifungal activity in a murine model of VVC were evaluated. Nanoparticles showed 147.6 ± 16.7 nm of diameter, 0.301 ± 0.09 of polydispersity index, - 29.9 ± 3.76 mV of zeta potential, and 87.27 % of encapsulation efficiency. They released about 81 % of AMP in 96 h; and provided the elimination of 100 % of the vaginal fungal burden in 24 h. It was suggested that the AMP EUD nanoparticles/HA penetrated into the vaginal epithelium via CD44 receptors. These AMP EUD nanoparticles/HA represent a non-conventional vaginal formulation to improve the treatment of VVC.