Browsing by Author "Pacheco, Richard de Campos"

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    Rickettsia in synanthropic and domestic animals and in their host from two areas of low endemicity for Brazilian spotted fever, in the eastern region of Minas Gerais state, Brazil.
    (2010) Milagres, Bruno Silva; Padilha, Amanda de Freitas; Gabriel G. Gomes; Montandon, Carlos Emmanuel; Pena, Dárlen Crísthiê Hermelinda; Bastos, Fernanda Aparecida Nieri; Silveira, Iara; Pacheco, Richard de Campos; Labruna, Marcelo Bahia; Bouyer, Donald H.; Freitas, Renata Nascimento de; Walker, David Hughes; Mafra, Cláudio Lísias; Galvão, Márcio Antônio Moreira
    The aim of this study was to understand the current epidemiology of rickettsial diseases in two rickettsialendemic regions in Brazil. In the municipalities of Pingo D’Agua and Santa Cruz do Escalvado, among serum samples obtained from horses and dogs, reactivity by immunofluorescent assay against spotted fever group rickettsiae was verified. In some serum samples from opossums ( Didelphis aurita ) captured in Santa Cruz do Escalvado, serologic response against rickettsiae was also verified. Polymerase chain reaction identified rickettsiae only in ticks and fleas obtained in Santa Cruz do Escalvado. Rickettsiae in samples had 100% sequence homology with Rickettsia felis . These results highlight the importance of marsupials in maintenance of the sylvatic cycle of rickettsial disease and potential integration with the domestic cycle. Our data also support the importance of horses and dogs as sentinels in monitoring circulation of rickettsiae in an urban area.
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    Spotted fever group rickettsia in small rodents from areas of low endemicity for brazilian spotted fever in the eastern region of Minas Gerais state, Brazil.
    (2013) Milagres, Bruno Silva; Padilha, Amanda de Freitas; Montandon, Carlos Emmanuel; Freitas, Renata Nascimento de; Pacheco, Richard de Campos; Walker, David Hughes; Labruna, Marcelo Bahia; Mafra, Cláudio Lísias; Galvão, Márcio Antônio Moreira
    We investigated the humoral immune response against different species of Rickettsia in serum samples from small rodents collected in two areas of a silent focus for Brazilian spotted fever in the eastern region of Minas Gerais State, Brazil. Sera samples were analyzed by indirect immunofluorescence assay using antigens from Rickettsia species of the spotted fever, ancestral, and transition groups. Titers ³ 1:64 were considered positive. In Santa Cruz do Escalvado, 94% (30 of 32) of the samples collected from Rattus rattus, 22% (5 of 23) from Nectomys squamipes, and 80% (4 of 5) from Akodon sp., reacted by indirect immunofluorescence assay with Rickettsia antigens of the spotted fever group. In the municipality of Pingo D’A´ gua, 84% (26 of 31) of the samples collected from R. rattus, 86% (6 of 7) of the samples from Oryzomys subflavus, 86% (6 of 7) from N. squamipes, and 100% (1 of 1) from Bolomys sp. contained antibodies that reacted with rickettsial antigens of the spotted fever group. These results demonstrated the previous exposure of small rodents to spotted fever group Rickettsia, suggesting the participation of these animals in the natural history of these rickettsiae in this region.