Browsing by Author "Sampaio, Kinulpe Honorato"

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    Biodegradable seeds of holmium don´t change neurological function after implant in brain of rats.
    (2017) Diniz, Mirla Fiuza; Ferreira, Diogo Milioli; Lima, Wanderson Geraldo de; Pedrosa, Maria Lúcia; Silva, Marcelo Eustáquio; Araujo, Stanley de Almeida; Sampaio, Kinulpe Honorato; Campos, Tarcísio Passos Ribeiro de; Siqueira, Sávio Lana
    Aim: To evaluate the surgical procedure and parenchymal abnormalities related to implan-tation of ceramic seeds with holmium-165 in rats’ brain.Background: An effective method of cancer treatment is brachytherapy in which radioactiveseeds are implanted in the tumor, generating a high local dose of ionizing radiation thatcan eliminate tumor cells while protecting the surrounding healthy tissue. BiodegradableHo166-ceramic-seeds have been addressed recently.Methods and materials: The experiments in this study were approved by the Ethics Com-mittee on Animal Use at the Federal University of Ouro Preto, protocol number 2012/034.Twenty-one adult Fischer rats were divided into Naive Group, Sham Group and Group forseed implants (ISH). Surgical procedures for implantation of biodegradable seeds were doneand 30 days after the implant radiographic examination and biopsy of the brain were per-formed. Neurological assays were also accomplished to exclude any injury resulting fromeither surgery or implantation of the seeds.Results: Radiographic examination confirmed the location of the seeds in the brain. Neuro-logical assays showed animals with regular spontaneous activity. The histological analysisshowed an increase of inflammatory cells in the brain of the ISH group. Electron microscopyevidenced cytoplasmic organelles to be unchanged. Biochemical analyzes indicate there was neither oxidative stress nor oxidative damage in the ISH brain. CAT activity showed no difference between the groups as well as lipid peroxidation measured by TBARS. Conclusions: The analysis of the data pointed out that the performed procedure is safe as no animal showed alterations of the neurological parameters and the seeds did not promote histological architectural changes in the brain tissue.
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    Physical activity prevents alterations in mitochondrial ultrastructure and glucometabolic parameters in a high-sugar diet model.
    (2017) Queiroz, Karina Barbosa de; Sampaio, Kinulpe Honorato; Rossoni Júnior, Joamyr Victor; Leal, Diego Andrade; Pinto, Angélica Barbosa Gonçalves; Oliveira, Lenice Kappes Becker; Evangelista, Elísio Alberto; Cota, Renata Guerra de Sá
    Endurance exercise is a remarkable intervention for the treatment of many diseases. Mitochondrial changes on skeletal muscle are likely important for many of the benefits provided by exercise. In this study, we aimed to evaluate the effects that a regular physical activity (swimming without workload) has on mitochondrial morphological alterations and glucometabolic parameters induced by a high-sugar diet (HSD). Weaned male Wistar rats fed with a standard diet or a HSD (68% carbohydrate) were subjected to 60 minutes of regular physical activity by swimming (without workload) for four- (20 sessions) or eight-week (40 sessions) periods. After training, animals were euthanized and the sera, adipose tissues, and skeletal muscles were collected for further analysis. The HSD increased body weight after an 8- week period; it also increased the fat pads and the adipose index, resulting in glucose intolerance and insulin resistance (IR). Transmission electron microscopy showed an increase in alterations of mitochondrial ultrastructure in the gastrocnemius muscle, as well as a decrease in superoxide dismutase (SOD) activity, and an increase in protein carbonylation. Regular physical activity partially reverted these alterations in rats fed a HSD, preventing mitochondrial morphological alterations and IR. Moreover, we observed a decrease in Pgc1α expression (qPCR analysis) in STD-EXE group and a less pronounced reduction in HSD-EXE group after an 8-week period. Thus, regular physical activity (swimming without workload) in rats fed a HSD can prevent mitochondrial dysfunction and IR, highlighting the crucial role for physical activity on metabolic homeostasis.
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    Polyomavirus infection in renal allograft.
    (2013) Araújo, Stanley de Almeida; Sampaio, Kinulpe Honorato; Fernandes Júnior, Ladislau José