Browsing by Author "Vicente, Ana Carolina Paulo"

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    Outbreaks of cholera-like diarrhoea caused by enterotoxigenic escherichia coli in the Brazilian Amazon rainforest.
    (2005) Vicente, Ana Carolina Paulo; Teixeira, Luiz Fernando de Medeiros; Iniguez Rojas, Luiza; Gomes, Maria das Graças de Luna; Silva, Luciete Almeida; Andrade, João Ramos Costa; Guth, Beatriz Ernestina Cabilio
    The relationship between enteropathogens and severe diarrhoea in the Brazilian Amazon is poorly understood. In 1998, outbreaks of acute diarrhoea clinically diagnosed as cholera occurred in two small villages localized far from the main cholera route in the Brazilian rainforest. PCR was performed on some enteropathogens and heat-labile (LT) and/or heat-stable (STh) toxin genes, the virulence determinants of enterotoxigenic Escherichia coli (ETEC), were detected. Further characterization of ETEC isolates revealed the presence of two clones, one from each outbreak. One presenting serotype O167:H5 harboured LT-I and STh toxin genes and expressed the CS5CS6 colonization factor. The other, a non-typeable serotype, was positive for the LT-I gene and expressed the CS7 colonization factor. The current study demonstrates the importance of molecular diagnosis in regions such as the Amazon basin, where the enormous distances and local support conditions make standard laboratory diagnosis difficult. Here we also show that the mis-identified cholera cases were in fact associated with ETEC strains. This is the first report of ETEC, molecularly characterized as the aetiological agent of severe diarrhoea in children and adults in the Brazilian Amazon Rainforest. © 2005 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.
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    Vibrio mimicus from Vibrio cholerae by multilocus enzyme electrophoresis.
    (2001) Vieira, Verônica V.; Teixeira, Luiz Fernando de Medeiros; Vicente, Ana Carolina Paulo; Momen, Hooman; Salles, Carlos André
    In this study, we demonstrated that analyzed strains of Vibrio mimicus and Vibrio cholerae could be separated in two groups by using multilocus enzyme electrophoresis (MEE) data from 14 loci. We also showed that the combination of four enzymatic loci enables us to differentiate these two species. Our results showed that the ribosomal intergenic spacer regions PCR-mediated identification system failed, in some cases, to differentiate between V. mimicus and V. cholerae. On the other hand, MEE proved to be a powerful molecular tool for the discrimination of these two species even when atypical strains were analyzed.