High-through identification of T cell-specific phage-exposed mimotopes using PBMCs from tegumentary leishmaniasis patients and their use as vaccine candidates against Leishmania amazonensis infection.

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dc.contributor.authorCarvalho, Gerusa Brandão de
dc.contributor.authorCosta, Lourena Emanuele
dc.contributor.authorLage, Daniela Pagliara
dc.contributor.authorRamos, Fernanda Fonseca
dc.contributor.authorSantos, Thaís Teodoro de Oliveira
dc.contributor.authorRibeiro, Patrícia Aparecida Fernandes
dc.contributor.authorDias, Daniel Silva
dc.contributor.authorSalles, Beatriz Cristina Silveira
dc.contributor.authorLima, Mariana Pedrosa
dc.contributor.authorCarvalho, Lívia Mendes
dc.contributor.authorDias, Ana C. S.
dc.contributor.authorAlves, Patrícia Terra
dc.contributor.authorFranklin, Michelle Lucrécio
dc.contributor.authorSilva, Renata A. M.
dc.contributor.authorDuarte, Mariana Costa
dc.contributor.authorSouza, Daniel Menezes
dc.contributor.authorRoatt, Bruno Mendes
dc.contributor.authorChávez Fumagalli, Miguel Angel
dc.contributor.authorGoulart Filho, Luiz Ricardo
dc.contributor.authorTeixeira Junior, Antonio Lucio
dc.contributor.authorCoelho, Eduardo Antônio Ferraz
dc.date.accessioned2019-04-02T18:14:24Z
dc.date.available2019-04-02T18:14:24Z
dc.date.issued2019
dc.description.abstractIn the current study, phage-exposed mimotopes as targets against tegumentary leishmaniasis (TL) were selected by means of bio-panning cycles employing sera of TL patients and healthy subjects, besides the immune stimulation of peripheral blood mononuclear cells (PBMCs) collected from untreated and treated TL patients and healthy subjects. The clones were evaluated regarding their specific interferon-γ (IFN-γ) and interleukin-4 (IL-4) production in the in vitro cultures, and selectivity and specificity values were calculated, and those presenting the best results were selected for the in vivo experiments. Two clones, namely A4 and A8, were identified and used in immunization protocols from BALB/c mice to protect against Leishmania amazonensis infection. Results showed a polarized Th1 response generated after vaccination, being based on significantly higher levels of IFN-γ, IL-2, IL-12, tumour necrosis factor-α (TNF-α) and granulocyte-macrophage colony-stimulating factor (GM-CSF); which were associated with lower production of specific IL-4, IL-10 and immunoglobulin G1 (IgG1) antibodies. Vaccinated mice presented significant reductions in the parasite load in the infected tissue and distinct organs, when compared with controls. In conclusion, we presented a strategy to identify new mimotopes able to induce Th1 response in PBMCs from TL patients and healthy subjects, and that were successfully used to protect against L. amazonensis infectionpt_BR
dc.identifier.citationCARVALHO, G. B. de et al. High-through identification of T cell-specific phage-exposed mimotopes using PBMCs from tegumentary leishmaniasis patients and their use as vaccine candidates against Leishmania amazonensis infection. Parasitology, v. 146, n. 3, p. 322-332, mar. 2019. Disponível em: <https://www.cambridge.org/core/journals/parasitology/article/highthrough-identification-of-t-cellspecific-phageexposed-mimotopes-using-pbmcs-from-tegumentary-leishmaniasis-patients-and-their-use-as-vaccine-candidates-against-leishmania-amazonensis-infection/71AD5ADD21870A5C7D684E0E6D512E9D>. Acesso em: 22 fev. 2019.pt_BR
dc.identifier.issn00311820
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/10909
dc.identifier.uri2https://www.cambridge.org/core/journals/parasitology/article/highthrough-identification-of-t-cellspecific-phageexposed-mimotopes-using-pbmcs-from-tegumentary-leishmaniasis-patients-and-their-use-as-vaccine-candidates-against-leishmania-amazonensis-infection/71AD5ADD21870A5C7D684E0E6D512E9Dpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectImmune responsept_BR
dc.subjectPhage displaypt_BR
dc.subjectPeripheral blood mononuclear cellspt_BR
dc.titleHigh-through identification of T cell-specific phage-exposed mimotopes using PBMCs from tegumentary leishmaniasis patients and their use as vaccine candidates against Leishmania amazonensis infection.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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