Development of a label-free immunosensor based on surface plasmon resonance technique for the detection of anti-Leishmania infantum antibodies in canine serum.

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dc.contributor.authorSouto, Dênio Emanuel Pires
dc.contributor.authorSilva, Jussara Vieira
dc.contributor.authorMartins, Helen Rodrigues
dc.contributor.authorReis, Alexandre Barbosa
dc.contributor.authorLuz, Rita de Cássia Silva
dc.contributor.authorKubota, Lauro Tatsuo
dc.contributor.authorDamos, Flavio Santos
dc.date.accessioned2015-01-21T18:13:02Z
dc.date.available2015-01-21T18:13:02Z
dc.date.issued2013
dc.description.abstractIn this work ,a surface Plasmon resonance (SPR) immune sensor was developed using an 11-mercaptoundecanoic acid (11-MUA) modified gold SPR sensor chip for the detection of anti- Leishmania infantum antibodies. The soluble antigens of L. infantum were securely immobilized on an SPR gold disk byan 11-MUA self-assembled monolayer. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electrochemical microscopy (SECM) techniques were employed in the characterization of the antigenim mobilization. After the immune sensor construction, canine serum positive for visceral leishmaniasis was added to its surface and showed significant variation in the SPR angle, indicating excellent sensitivity of the technique for antigen–antibody interaction detection. Moreover, the addition of negative serum was accompanied by as maller response, demonstrating that the immunosensor shows good specificity against anti-L. infantum antibodies. Therefore, this work demonstrates the success ful development of an SPR sensor for anti- L. infantum antibodies detection in short time, showing a great perspective as asensing system of visceral leishmania sisinende micregions.pt_BR
dc.identifier.citationSOUTO, D. E. P. et al. Development of a label-free immunosensor based on surface plasmon resonance technique for the detection of anti-Leishmania infantum antibodies in canine serum. Nutrition Research, v. 32, n. 9, p. 709-717, set. 2012. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0271531712001479>. Acesso em: 11 jun. 2019.
dc.identifier.doihttps://doi.org/10.1016/j.nutres.2012.06.020
dc.identifier.issn0956-5663
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4311
dc.language.isoen_USpt_BR
dc.rights.licenseO periódico Biosensor and Bioeletronics concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3440301034509.pt_BR
dc.subjectLeishmania infantumpt_BR
dc.subjectVisceral leishmaniasispt_BR
dc.subjectPlasmon resonancept_BR
dc.titleDevelopment of a label-free immunosensor based on surface plasmon resonance technique for the detection of anti-Leishmania infantum antibodies in canine serum.en
dc.typeArtigo publicado em periodicopt_BR
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