Trypanosoma cruzi nucleoside triphosphate diphosphohydrolase 1 (TcNTPDase-1) biochemical characterization, immunolocalization and possible role in host cell adhesion.

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dc.contributor.authorMoura, Christiane Mariotini
dc.contributor.authorBastos, Matheus Silva e
dc.contributor.authorCastro, Felipe Freitas de
dc.contributor.authorTrindade, Mellina Lanna
dc.contributor.authorVasconcellos, Raphael de Souza
dc.contributor.authorVale, Myriam Augusta Araújo Neves do
dc.contributor.authorMoreira, Bernardo Pereira
dc.contributor.authorSantos, Ramon de Freitas
dc.contributor.authorOliveira, Claudia Miranda de
dc.contributor.authorCunha, Luana Celina Seraphim
dc.contributor.authorSouto, Xênia Macedo
dc.contributor.authorBressan, Gustavo Costa
dc.contributor.authorSilva Júnior, Abelardo
dc.contributor.authorBaqui, Munira Muhammad Abdel
dc.contributor.authorBahia, Maria Terezinha
dc.contributor.authorAlmeida, Márcia Rogéria de
dc.contributor.authorFernandes, José Roberto Meyer
dc.contributor.authorFietto, Juliana Lopes Rangel
dc.date.accessioned2015-04-01T17:36:43Z
dc.date.available2015-04-01T17:36:43Z
dc.date.issued2014
dc.description.abstractPrevious work has suggested that Trypanosoma cruzi diphosphohydrolase 1 (TcNTPDase-1) may be involved in the infection of mammalian cells and serve as a potential target for rational drug design. In this work, we produced recombinant TcNTPDase-1 and evaluated its nucleotidase activity, cellular localiza- tion and role in parasite adhesion to mammalian host cells. TcNTPDase-1 was able to utilize a broad range of triphosphate and diphosphate nucleosides. The enzyme’s Km for ATP (0.096mM) suggested a capabil-ity to influence the host’s ATP-dependent purinergic signaling. The use of specific polyclonal antibodies allowed us to confirm the presence of TcNTPDase-1 at the surface of parasites by confocal and electron microscopy. In addition, electron microscopy revealed that TcNTPDase-1 was also found in the flagellum, flagellum insertion region, kinetoplast, nucleus and intracellular vesicles. The presence of this enzyme in the flagellum insertion region and vesicles suggests that it may have a role in nutrient acquisition, and the widespread distribution of TcNTPDase-1 within the parasite suggests that it may be involved in other biological process. Adhesion assays using anti-TcNTPDase-1 polyclonal antibodies as a blocker or purified recombinant TcNTPDase-1 as a competitor revealed that the enzyme has a role in parasite–host cell adhesion. These data open new frontiers to future studies on this specific parasite–host interaction and other unknown functions of TcNTPDase-1 related to its ubiquitous localization.pt_BR
dc.identifier.citationMOURA, C. M. et al. Trypanosoma cruzi nucleoside triphosphate diphosphohydrolase 1 (TcNTPDase-1) biochemical characterization, immunolocalization and possible role in host cell adhesion. Acta Tropica, v. 130, p. 140-147, 2014. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X13003318>. Acesso em: 08 nov. 2014.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.actatropica.2013.11.008
dc.identifier.issn0001-706X
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4931
dc.language.isoen_USpt_BR
dc.rights.licenseThis article is published under the terms of the Creative Commons Attribution-NonCommercial-No Derivatives License (CC BY NC ND). Fonte: o próprio artigo.pt_BR
dc.subjectRecombinant proteinpt_BR
dc.titleTrypanosoma cruzi nucleoside triphosphate diphosphohydrolase 1 (TcNTPDase-1) biochemical characterization, immunolocalization and possible role in host cell adhesion.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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