Interleukin-6 actions in the hypothalamus protects against obesity and is involved in the regulation of neurogenesis.

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dc.contributor.authorBóbbo, Vanessa Cristina Dias
dc.contributor.authorEngel, Daiane Fátima
dc.contributor.authorJara, Carlos Poblete
dc.contributor.authorMendes, Natália Ferreira
dc.contributor.authorTovolli, Roberta Haddad
dc.contributor.authorPrado, Thais P.
dc.contributor.authorOliveira, Davi Sidarta
dc.contributor.authorMorari, Joseane
dc.contributor.authorVelloso, Licio Augusto
dc.contributor.authorAraújo, Eliana Pereira
dc.date.accessioned2022-12-16T19:55:24Z
dc.date.available2022-12-16T19:55:24Z
dc.date.issued2021pt_BR
dc.description.abstractBackground: Interleukin-6 (IL6) produced in the context of exercise acts in the hypothalamus reducing obesity- associated inflammation and restoring the control of food intake and energy expenditure. In the hippocampus, some of the beneficial actions of IL6 are attributed to its neurogenesis-inducing properties. However, in the hypothalamus, the putative neurogenic actions of IL6 have never been explored, and its potential to balance energy intake can be an approach to prevent or attenuate obesity. Methods: Wild-type (WT) and IL6 knockout (KO) mice were employed to study the capacity of IL6 to induce neurogenesis. We used cell labeling with Bromodeoxyuridine (BrdU), immunofluorescence, and real-time PCR to determine the expression of markers of neurogenesis and neurotransmitters. We prepared hypothalamic neuroprogenitor cells from KO that were treated with IL6 in order to provide an ex vivo model to further characterizing the neurogenic actions of IL6 through differentiation assays. In addition, we analyzed single-cell RNA sequencing data and determined the expression of IL6 and IL6 receptor in specific cell types of the murine hypothalamus. Results: IL6 expression in the hypothalamus is low and restricted to microglia and tanycytes, whereas IL6 receptor is expressed in microglia, ependymocytes, endothelial cells, and astrocytes. Exogenous IL6 reduces diet-induced obesity. In outbred mice, obesity-resistance is accompanied by increased expression of IL6 in the hypothalamus. IL6 induces neurogenesis-related gene expression in the hypothalamus and in neuroprogenitor cells, both from WT as well as from KO mice. Conclusion: IL6 induces neurogenesis-related gene expression in the hypothalamus of WT mice. In KO mice, the neurogenic actions of IL6 are preserved; however, the appearance of new fully differentiated proopiomelanocortin (POMC) and neuropeptide Y (NPY) neurons is either delayed or disturbed.pt_BR
dc.identifier.citationBÓBBO, V. C. D. et al. Interleukin-6 actions in the hypothalamus protects against obesity and is involved in the regulation of neurogenesis. Journal of Neuroinflammation, v. 18, 2021. Disponível em: <https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-021-02242-8>. Acesso em: 11 out. 2022.pt_BR
dc.identifier.doihttps://doi.org/10.1186/s12974-021-02242-8pt_BR
dc.identifier.issn1742-2094
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/15921
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the materia. Fonte: o PDF do artigo.pt_BR
dc.subjectCytokinept_BR
dc.subjectMetabolismpt_BR
dc.subjectNeuronpt_BR
dc.subjectAstrocytept_BR
dc.subjectBrainpt_BR
dc.titleInterleukin-6 actions in the hypothalamus protects against obesity and is involved in the regulation of neurogenesis.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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