The role of immunohistochemistry in the detection of vascular invasion in specimens of endoscopic submucosal dissection.

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dc.contributor.authorAldeman, Nayze Lucena Sangreman
dc.contributor.authorPalhares, Daniel Moore Freitas
dc.contributor.authorAraújo, Stanley de Almeida
dc.contributor.authorPedrosa, Moisés Salgado
dc.contributor.authorCastro, Luísa Lima
dc.contributor.authorArantes, Vitor Nunes
dc.contributor.authorCabral, Mônica Maria Demas Álvares
dc.date.accessioned2016-01-26T15:52:40Z
dc.date.available2016-01-26T15:52:40Z
dc.date.issued2013
dc.description.abstractIntroduction: Endoscopic submucosal dissection (ESD) of early neoplasias of the gastrointestinal tract (GIT) has been increasingly applied as an alternative to invasive surgical procedures, with the aim to preserve the patient’s organ and quality of life, although it does not allow the histopathological analysis of lymph nodes. Previous studies demonstrated that the presence of neoplastic emboli in lymphatic (lymphatic vascular invasion [LVI]) or blood vessels (blood vascular invasion [BVI]) is considered a positive predictive factor for the occurrence of lymph node metastasis. The assessment of vascular invasion carried out only by routine hematoxylin and eosin staining (HE) may yield both falsepositive and false-negative results. D2-40 is a specific monoclonal antibody to the lymphatic endothelium. Thus, it is useful for identifying LVI and distinguishing if tumor embolization is found in blood or lymphatic vessels. Objective: To determine the role of immunohistochemistry (IHC) in the assessment of ESD specimens by comparing the detection of LVI and BVI by HE and IHC with D2-40 and CD34 immunolabeling. Method: We conducted the IHC study using D2-40 and CD34 markers (pan-endothelial) in 30 cases of ESD with histological diagnosis of carcinoma in order to assess the presence of LVI and BVI. Results: The detection of LVI was more prevalent than BVI. Three out of six cases with LVI were false-positive by HE and six were false-negative by IHC. Regarding BVI, five cases were identified and one was false-negative by IHC. Conclusion: Our results indicated that the histopathological analysis of ESD specimens by exclusively routine HE staining does not allow proper evaluation of BVI or LVI.pt_BR
dc.identifier.citationALDEMAN, N. L. S. et al. The role of immunohistochemistry in the detection of vascular invasion in specimens of endoscopic submucosal dissection. Jornal Brasileiro de Patologia e Medicina Laboratorial, v. 49, p. 273-277, 2013. Disponível em: <http://www.scielo.br/pdf/jbpml/v49n4/v49n4a08.pdf>. Acesso em: 21 out. 2015.pt_BR
dc.identifier.doihttp://dx.doi.org/10.1590/S1676-24442013000400008
dc.identifier.issn1678-4774
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/6223
dc.language.isoen_USpt_BR
dc.rights.licenseTodo o conteúdo do periódico Jornal Brasileiro de Patologia e Medicina Laboratorial, exceto onde identificado, está sob uma licença Creative Commons que permite copiar, distribuir e transmitir o trabalho em qualquer suporte ou formato desde que sejam citados o autor e o licenciante. Não permite o uso para fins comerciais. Fonte: Jornal Brasileiro de Patologia e Medicina Laboratorial <http://www.scielo.br/scielo.php?script=sci_serial&pid=1676-2444&lng=en&nrm=iso>. Acesso em: 28 ago. 2019.pt_BR
dc.subjectLymphatic vascular invasionpt_BR
dc.subjectBlood vascular invasionpt_BR
dc.titleThe role of immunohistochemistry in the detection of vascular invasion in specimens of endoscopic submucosal dissection.pt_BR
dc.title.alternativeO papel da imuno-histoquímica na detecção de invasão vascular em espécimes de dissecção endoscópica da submucosa.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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