Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi.

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dc.contributor.authorCardoso, Josiane
dc.contributor.authorLima, Carla de Paula
dc.contributor.authorLeal, Tiago Ferreira
dc.contributor.authorGradia, Daniela Fiori
dc.contributor.authorFragoso, Stênio Perdigão
dc.contributor.authorGoldenberg, Samuel
dc.contributor.authorCota, Renata Guerra de Sá
dc.contributor.authorKrieger, Marco Aurélio
dc.date.accessioned2015-03-20T19:05:43Z
dc.date.available2015-03-20T19:05:43Z
dc.date.issued2011
dc.description.abstractProteasomes are large protein complexes, whose main function is to degrade unnecessary or damaged proteins. The inhibition of proteasome activity in Trypanosoma cruzi blocks parasite replication and cellular differentiation. We demonstrate that proteasome-dependent proteolysis occurs during the cellular differentiation of T. cruzi from replicative non-infectious epimastigotes to non-replicative and infectious trypomastigotes (metacyclogenesis). No peaks of ubiquitinmediated degradation were observed and the profile of ubiquitinated conjugates was similar at all stages of differentiation. However, an analysis of carbonylated proteins showed significant variation in oxidized protein levels at the various stages of differentiation and the proteasome inhibition also increased oxidized protein levels. Our data suggest that different proteasome complexes coexist during metacyclogenesis. The 20S proteasome may be free or linked to regulatory particles (PA700, PA26 and PA200), at specific cell sites and the coordinated action of these complexes would make it possible for proteolysis of ubiquitin-tagged proteins and oxidized proteins, to coexist in the cell.pt_BR
dc.identifier.citationCARDOSO, J. et al. Analysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi. Plos One, v. 6, p. e21027, 2011. Disponível em: <http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021027>. Acesso em: 08 nov. 2014.pt_BR
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0021027
dc.identifier.issn1932-6203
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4715
dc.language.isoen_USpt_BR
dc.rights.licenseOs trabalhos publicados na Plos one estão sob Licença Creative Commons que permite copiar, distribuir e transmitir o trabalho, desde que sejam citados o autor e licenciante. Não permite o uso para fins comerciais nem a adaptação. Fonte: Plos one <https://www.plos.org/open-access>. Acesso em: 03 jan. 2017.pt_BR
dc.titleAnalysis of proteasomal proteolysis during the in vitro metacyclogenesis of Trypanosoma cruzi.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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