In vitro Infectivity of strains isolated from dogs naturally infected with Leishmania infantum present a distinct pathogenic profile in hamsters.

Simple item page
dc.contributor.authorResende, Lucilene Aparecida
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar
dc.contributor.authorMoreira, Nádia das Dores
dc.contributor.authorFerreira, Sidney de Almeida
dc.contributor.authorLanna, Mariana Ferreira
dc.contributor.authorCardoso, Jamille Mirelle de Oliveira
dc.contributor.authorMathias, Fernando Augusto Siqueira
dc.contributor.authorVital, Wendel Coura
dc.contributor.authorMariano, Reysla Maria da Silveira
dc.contributor.authorLeite, Jaqueline Costa
dc.contributor.authorSilveira, Patricia
dc.contributor.authorCarvalho, Tatiane Furtado de
dc.contributor.authorSantos, Renato Lima
dc.contributor.authorLemos, Denise da Silveira
dc.contributor.authorMartins Filho, Olindo Assis
dc.contributor.authorDutra, Walderez Ornelas
dc.contributor.authorReis, Alexandre Barbosa
dc.contributor.authorGiunchetti, Rodolfo Cordeiro
dc.date.accessioned2021-09-03T15:05:10Z
dc.date.available2021-09-03T15:05:10Z
dc.date.issued2020pt_BR
dc.description.abstractVisceral leishmaniasis (VL) is a severe disease caused by Leishmania infantum. Dogs are the parasite’s main reservoir, favoring its transmission in the urban environment. The analysis of L. infantum from infected dogs contributes to the identification of more virulent parasites, thereby supporting basic and applied studies such as vaccinal and therapeutic strategies. We proposed the in vitro and in vivo characterization of L. infantum strains from naturally infected dogs from a VL endemic area based on an infectivity and pathogenicity analysis. DH82 canine macrophages were infected in vitro with different strains for infectivity analysis, showing distinct infectivity profiles. The strains that showed greater and lesser infectivity using in vitro analyses (616 and 614, respectively) were used to infect hamsters for pathogenicity analysis. The group infected with strain 616 showed 100% survival while the group infected with strain 614 showed 50% after seven months of follow up. Furthermore, the 614 strain induced more noticeable clinicopathological changes and biochemical abnormalities in liver function, along with high inflammation and parasite load in the liver and spleen. We confirmed high variability of infectivity and pathogenicity in L. infantum strains from infected dogs. The results support the belief that screening for L. infantum infectivity using in vitro experiments is inadequate when it comes to selecting the most pathogenic strain.pt_BR
dc.identifier.citationRESENDE, L. A. et al. In vitro Infectivity of strains isolated from dogs naturally infected with Leishmania infantum present a distinct pathogenic profile in hamsters. Frontiers in Medicine, v. 7, artigo 496, ago. 2020. Disponível em: <https://www.frontiersin.org/articles/10.3389/fmed.2020.00496/full>. Acesso em: 10 jun. 2021.pt_BR
dc.identifier.doihttps://doi.org/10.3389/fmed.2020.00496pt_BR
dc.identifier.issn2296-858X
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/13645
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Fonte: o PDF do artigo.pt_BR
dc.subjectVisceral leishmaniasispt_BR
dc.subjectExperimental infectionpt_BR
dc.subjectPathogenicitypt_BR
dc.titleIn vitro Infectivity of strains isolated from dogs naturally infected with Leishmania infantum present a distinct pathogenic profile in hamsters.pt_BR
dc.typeArtigo publicado em periodicopt_BR
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
ARTIGO_VitroInfectivityStrains.pdf
Size:
1.51 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
DEACL - Artigos publicados em periódicos