Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.

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dc.contributor.authorSouza, Ronny Francisco de
dc.contributor.authorSantos, Yaro Luciolo dos
dc.contributor.authorVasconcellos, Raphael de Souza
dc.contributor.authorPereira, Lucas Borges
dc.contributor.authorCaldas, Ivo Santana
dc.contributor.authorAlmeida, Márcia Rogéria de
dc.contributor.authorBahia, Maria Terezinha
dc.contributor.authorFietto, Juliana Lopes Rangel
dc.date.accessioned2015-04-01T17:36:03Z
dc.date.available2015-04-01T17:36:03Z
dc.date.issued2013
dc.description.abstractCanine visceral leishmaniasis is an important public health concern. In the epidemiological context of human visceral leishmaniasis, dogs are considered the main reservoir of Leishmania parasites; therefore, dogs must be epidemiologically monitored constantly in endemic areas. Furthermore, dog to human transmission has been correlated with emerging urbanization and increasing rates of leishmaniasis infection worldwide. Leishmania (Leishmania) infantum (L. chagasi) is the etiologic agent of visceral leishmaniasis in the New World. In this work, a new L. (L.) infantum (L. chagasi) recombinant antigen, named ATP diphosphohydrolase (rLic-NTPDase-2), intended for use in the immunodiagnosis of CVL was produced and validated. The extracellular domain of ATP diphosphohydrolase was cloned and expressed in the pET21b-Escherichia coli expression system. Indirect ELISA assays were used to detect the purified rLic-NTPDase-2 antigen using a standard canine sera library. This library contained CVL-positive samples, leishmaniasis-negative samples and samples from Trypanosoma cruzi-infected dogs. The results show a high sensitivity of 100% (95% CI = 92.60–100.0%) and a high specificity of 100% (95% CI = 86.77–100.0%), with a high degree of confidence (k = 1). These findings demonstrate the potential use of this recombinant protein in immune diagnosis of canine leishmaniasis and open the possibility of its application to other diagnostic approaches, such as immunochromatography fast lateral flow assays and human leishmaniasis diagnosis.pt_BR
dc.identifier.citationSOUZA, R. F. de et al. Recombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis. Acta Tropica, v. 125, p. 60-66, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0001706X12003166#>. Acesso em: 08 nov. 2014.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.actatropica.2012.09.011
dc.identifier.issn0001-706X
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4928
dc.language.isoen_USpt_BR
dc.rights.licenseO periódico Acta Tropica concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3544871302050.pt_BR
dc.subjectCanine leishmaniasispt_BR
dc.subjectImmunodiagnosispt_BR
dc.subjectRecombinant proteinpt_BR
dc.subjectEcto nucleoside triphosphatept_BR
dc.subjectDiphosphohydrolasept_BR
dc.titleRecombinant leishmania (Leishmania) infantum ecto-nucleoside triphosphate diphosphohydrolase NTPDase-2 as a new antigen in canine visceral leishmaniasis diagnosis.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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