Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity.
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Date
2017
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Abstract
The metabolism of ethanol occurs mainly in the liver, promoting increase of reactive oxygen species and
nitrogen, leading to redox imbalance. Therefore, antioxidants can be seen as an alternative to reestablish
the oxidizing/reducing equilibrium. The aim of this study was to evaluate the antioxidant and hepatoprotective effect of aqueous extract of Baccharis trimera (Less.) DC., Asteraceae, in a model of hepatotoxicity
induced by ethanol. The extract was characterized and in vitro tests were conducted in HepG2 cells. It was
evaluated the cells viability exposed to aqueous extract for 24 h, ability to scavenging the radical DPPH,
besides the production of reactive oxygen species and nitric oxide, and the influence on the transcriptional activity of transcription factor Nrf2 (12 and 24 h) after exposure to 200 mM ethanol. The results
showed that aqueous extract was non-cytotoxic in any concentration tested; moreover, it was observed
a decrease in ROS and NO production, also promoting the transcriptional activity of Nrf2. In vivo, we pretreatment male rats Fisher with 600 mg/kg of aqueous extract and 1 h later 5 ml/kg of absolute ethanol
was administrated. After two days of treatment, the animals were euthanized and lipid profile, hepatic
and renal functions, antioxidant status and oxidative damage were evaluated. The treatment with extract
improved liver function and lipid profile, reflecting the reduction of lipid microvesicules in the liver. It
also promoted an increase of glutathione peroxidase activity, decrease of oxidative damage and MMP2 activity. These results, analyzed together, suggest the hepatoprotective effect of B. trimera aqueous
extract.
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Keywords
Ethanol, Redox imbalance
Citation
RABELO, A. C. S. et al. Aqueous extract of Baccharis trimera improves redox status and decreases the severity of alcoholic hepatotoxicity. Revista Brasileira de Farmacognosia, v. 27, n. 6, p. 729-738, nov./dez. 2017. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0102695X17303678>. Acesso em: 22 fev. 2019.