Investigation on the 19S ATPase proteasome subunits (Rpt1 6) conservation and their differential gene expression in Schistosoma mansoni.

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dc.contributor.authorPereira Junior, Olavo dos Santos
dc.contributor.authorPereira, Roberta Verciano
dc.contributor.authorSilva, Camila Siqueira
dc.contributor.authorBorges, William de Castro
dc.contributor.authorCota, Renata Guerra de Sá
dc.contributor.authorCabral, Fernanda Janku
dc.contributor.authorSilva, Sérgio Henrique da
dc.contributor.authorSoares, Cláudia Sossai
dc.contributor.authorMorais, Enyara Rezende
dc.contributor.authorMoreira, Érika Bueno de Carvalho
dc.contributor.authorMagalhães, Lizandra Guidi
dc.contributor.authorPaula, Fabiana Martins de
dc.contributor.authorRodrigues, Vanderlei
dc.date.accessioned2017-06-22T18:18:41Z
dc.date.available2017-06-22T18:18:41Z
dc.date.issued2013
dc.description.abstractThe ubiquitin-proteasome system is responsible for degradation of the majority of intracellular proteins in eukaryotic cells. The 26S proteasome proteolytic complex is composed of a 20S core particle responsible for protein degradation and the 19S lid which plays a role in the recognition of polyubiquitinated substrates. The 19S regulatory particle (Rps) is composed of ATPase (Rpt) and non-ATPase (Rpn) subunits. In this study, we analyzed the expression profile of 19S Rpt subunits in the larvae and adult stage of the Schistosoma mansoni life cycle. Conventional reverse transcriptase polymerase chain reaction (RT-PCR) revealed that the majority of the 19S Rpt subunits amplified at the expected molecular masses for various investigated stages. In addition, SmRpt1, SmRpt2, and SmRpt6 transcript levels were increased in 3 h-cultured schistosomula and reasonably maintained until 5 h in culture, as revealed by qRT-PCR. Phylogenetic analysis of 19S Rpt subunits showed high structural conservation in comparison to other Rpt orthologues. The mRNA expression profile of 19S Rpt subunits did not correlate with 26S proteasome proteolytic activity as judged by a 14C-casein-degrading assay, in the early cultured schistosomula. Taken together, these results revealed a differential expression profile for 19S Rpt subunits whose transcript levels could not be directly associated to 26S proteasome activity.pt_BR
dc.identifier.citationPEREIRA JÚNIOR, O. dos S. et al. Investigation on the 19S ATPase proteasome subunits (Rpt1 6) conservation and their differential gene expression in Schistosoma mansoni. Parasitology Research, v. 112, p. 235-242, 2012. Disponível em: <https://link.springer.com/article/10.1007%2Fs00436-012-3130-4>. Acesso em: 23 fev. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.1007/s00436-012-3130-4
dc.identifier.issn1432-1955
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/8051
dc.identifier.uri2https://link.springer.com/article/10.1007%2Fs00436-012-3130-4pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.titleInvestigation on the 19S ATPase proteasome subunits (Rpt1 6) conservation and their differential gene expression in Schistosoma mansoni.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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