Hydrogen production by Enterobacter sp. LBTM 2 using sugarcane bagasse hemicellulose hydrolysate and a synthetic substrate : understanding and controlling toxicity.
No Thumbnail Available
Date
2021
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Sugars released by thermochemical pretreatment of lignocellulosic biomass
are possible substrate for hydrogen production. However, the major drawback for
bacterial fermentation is the toxicity of weak acids and furan derivatives normally
present in such substrate. This study aimed to investigate the metabolism involved in
hydrogen production by the isolate Enterobacter LBTM2 using 10, 20 and 30-fold diluted
synthetic (SH) and sugarcane bagasse hemicellulose (SBH) hydrolysates. In addition, the
effects of acetic acid, formic acid and furfural on the bacterial metabolism, as well as
detoxification of SBH with activated carbon and molecularly imprinted polymers on the
hydrogen production were assessed. The results showed the best hydrogen yield was 0.46
mmol H2
/mmol sugar for 20-times diluted SH, which was 2.3-times higher than obtained
in SBH experiments. Bacterial growth and hydrogen production were negatively affected
by 0.8 g/L of acetic acid when added alone, but were totally inhibited when formic acid
(0.4 g/L) and furfural (0.3 g/L) were also supplied. However the maximum hydrogen
production of SBH20 has duplicated when 3% of powdered activated carbon was added
to the SBH experiment. The results presented herein can be helpful in understanding
the bottlenecks in biohydrogen production and could contribute towards development
of lignocellulosic biorefinery.
Description
Keywords
Acetic acid, Activated carbon, Biorefinery, Fermentation, Molecularly imprinted polymers
Citation
RINCON, I. M. C. et al. Hydrogen production by Enterobacter sp. LBTM 2 using sugarcane bagasse hemicellulose hydrolysate and a synthetic substrate: understanding and controlling toxicity. Anais da Academia Brasileira de Ciências, v. 93, artigo e20201679, 2021. Disponível em: <https://www.scielo.br/j/aabc/a/bSk9JPSrBjpGj67HwB7t5wp/abstract/?lang=en>. Acesso em: 11 out. 2022.