Differential expression of proteins in genetically distinct Trypanosoma cruzi samples (TcI and TcII DTUs) isolated from chronic Chagas disease cardiac patients.

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dc.contributor.authorOliveira, Maykon Tavares de
dc.contributor.authorRúbio, Karina Taciana Santos
dc.contributor.authorNeves, Leandro Xavier
dc.contributor.authorToledo, Max Jean de Ornelas
dc.contributor.authorBorges, William de Castro
dc.contributor.authorLana, Marta de
dc.date.accessioned2019-04-01T14:35:49Z
dc.date.available2019-04-01T14:35:49Z
dc.date.issued2018
dc.description.abstractBackground Trypanosoma cruzi, a hemoflagellate protozoan parasite and the etiological agent of Chagas disease (CD), exhibits great genetic and biological diversity. Infected individuals may present clinical manifestations with different levels of severity. Several hypotheses have been proposed to attempt to correlate the diversity of clinical signs and symptoms to the genetic variability of T. cruzi. This work aimed to investigate the differential expression of proteins from two distinct genetic groups of T. cruzi (discrete typing units TcI and TcII), isolated from chronically infected individuals displaying the cardiac form of CD. For this purpose, epimastigote forms of the two isolates were cultured in vitro and the cells recovered for protein extraction. Comparative two-dimensional (2D) gel electrophoreses were performed and differentially expressed spots selected for identification by mass spectrometry, followed by database searching and protein categorization. Results The 2D electrophoretic profiles revealed the complex composition of the T. cruzi extracted proteome. Protein spots were distributed along the entire pH and molecular mass ranges attesting for the integrity of the protein preparations. In total, 46 differentially expressed proteins were identified present in 40 distinct spots found in the comparative gel analyses. Of these, 16 displayed upregulation in the gel from TcI-typed parasites and 24 appeared overexpressed in the gel from TcII-typed parasites. Functional characterization of differentially expressed proteins revealed major alterations associated with stress response, lipid and amino acid metabolism in parasites of the TcII isolate, whilst those proteins upregulated in the TcI sample were primarily linked to central metabolic pathways. Conclusions The comparative 2D-gel electrophoresis allowed detection of major differences in protein expression between two T. cruzi isolates, belonging to the TcI and TcII genotypes. Our findings suggest that patients displaying the cardiac form of the disease harbor parasites capable of exhibiting distinct proteomic profiles. This should be of relevance to disease prognosis and treatment.pt_BR
dc.identifier.citationOLIVEIRA, M. T. de et al. Differential expression of proteins in genetically distinct Trypanosoma cruzi samples (TcI and TcII DTUs) isolated from chronic Chagas disease cardiac patients. Parasites & Vectors, v. 11, p. 1-11, set. 2018. Disponível em: <https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-018-3181-1>. Acesso em: 21 fev. 2019.pt_BR
dc.identifier.issn17563305
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/10872
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Fonte: o próprio artigo.pt_BR
dc.subjectDifferential proteomicspt_BR
dc.titleDifferential expression of proteins in genetically distinct Trypanosoma cruzi samples (TcI and TcII DTUs) isolated from chronic Chagas disease cardiac patients.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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