Fast determination of benzodiazepines in human urine via liquid-liquid extraction with low temperature partitioning and LC-HRMS.

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dc.contributor.authorMagalhães, Elisângela Jaqueline
dc.contributor.authorNascentes, Clésia Cristina
dc.contributor.authorAugusti, Rodinei
dc.contributor.authorQueiroz, Maria Eliana Lopes Ribeiro de
dc.contributor.authorSilva, Júlio César Cardoso da
dc.contributor.authorAfonso, Robson José de Cássia Franco
dc.date.accessioned2015-04-17T15:41:33Z
dc.date.available2015-04-17T15:41:33Z
dc.date.issued2012
dc.description.abstractA simple and high-throughput method to simultaneously determine selected benzodiazepines (i.e., diazepam, lorazepam, clonazepam, and bromazepam) in urine was developed and validated. The entire methodology consisted of the applica-tion of an innovative extraction/cleanup procedure, namely liquid-liquid extraction with low-temperature partitioning (LLE-LTP), and analysis by liquid chromatography combined with high-resolution mass spectrometry (LC-HRMS). The LLE-LTP procedure was optimized via factorial design and by evaluating crucial variables, specifically the freez-ing mode (either slow or fast), the urine/acetonitrile volume ratio, and the sample ionic strength. The benzodiazepines were quantified using matrix-matched calibration curves where the following parameters were assessed by validation protocol: in general, linearity range of 17 - 200 μg•L–1 (r > 0.9957); limits of detection lower than 5 μg•L–1; relative standard deviations (RSD) lower than 12.5%; and accuracy ranging from 72.3% to 117%. To test this procedure’s per-formance, the method was applied to determine the content of diazepam in actual urine samples. The validation results obtained for the method demonstrated that the present methodology could be potentially applied in proficient laborato-ries as a routine approach for determining benzodiazepines compounds content in urine.pt_BR
dc.identifier.citationMAGALHÃES, E. J. et al. Fast determination of benzodiazepines in human urine via liquid-liquid extraction with low temperature partitioning and LC-HRMS. American Journal of Analytical Chemistry, v. 03, p. 118-124, 2012. Disponível em: <http://www.scirp.org/journal/PaperInformation.aspx?PaperID=17285#disqusinfor>. Acesso em: 02 fev. 2015.pt_BR
dc.identifier.doihttp://dx.doi.org/10.4236/ajac.2012.32017
dc.identifier.issn2156-8278
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/5140
dc.language.isoen_USpt_BR
dc.rights.licenseThis work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License. Fonte: American Journal of Analytical Chemistry <http://www.scirp.org/journal/PaperInformation.aspx?PaperID=17285#disqusinfor>. Acesso em: 05 de fev. 2015.pt_BR
dc.subjectBenzodiazepinespt_BR
dc.subjectUrinept_BR
dc.subjectExtraction procedurept_BR
dc.subjectLow-temperature partitioningpt_BR
dc.titleFast determination of benzodiazepines in human urine via liquid-liquid extraction with low temperature partitioning and LC-HRMS.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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