IL-10 receptor blockade controls the in vitro infectivity of Leishmania infantum and promotes a Th1 activation in PBMC of dogs with visceral leishmaniasis.

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dc.contributor.authorCardoso, Jamille Mirelle de Oliveira
dc.contributor.authorBrito, Rory Cristiane Fortes de
dc.contributor.authorCosta, Ana Flávia Pereira
dc.contributor.authorMathias, Fernando Augusto Siqueira
dc.contributor.authorReis, Levi Eduardo Soares
dc.contributor.authorVieira, João Filipe Pereira
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar
dc.contributor.authorReis, Alexandre Barbosa
dc.contributor.authorRoatt, Bruno Mendes
dc.date.accessioned2023-01-26T19:16:54Z
dc.date.available2023-01-26T19:16:54Z
dc.date.issued2021pt_BR
dc.description.abstractAn important strategy to reduce the risk of visceral leishmaniasis (VL) in humans is to control the infection and disease progression in dogs, the domestic reservoir of Leishmania infantum parasites. Certain therapeutic strategies that modulate the host immune response show great potential for the treatment of experimental VL, restoring the impaired effector functions or decreasing host excessive responses. It is known that the overproduction of interleukin-10 (IL-10) promotes parasite replication and disease progression in human VL as well as in canine visceral leishmaniasis (CVL). Thus, in the present study we investigated the potential of the anticanine IL-10 receptor-blocking monoclonal antibody (Bloq IL-10R) to control and reduce in vitro infectivity of L. infantum and improve the ability of PBMC isolated from VL dogs to alter the lymphoproliferative response and intracytoplasmic cytokines. Overall, GFP+ Leishmania showed lower capacity of in vitro infectivity in the presence of Bloq IL-10R. Moreover, addition of Bloq IL-10R in cultured PBMC enhanced T-CD4 and CD8 proliferative response and altered the intracytoplasmic cytokine synthesis, reducing CD4+IL-4+ cells and increasing CD8+IFNγ+ cells after specific antigen stimulation in PBMC of dogs. Furthermore, we observed an increase of TNF-α levels in supernatant of cultured PBMC under IL-10R neutralizing conditions. Together, our findings are encouraging and reaffirm an important factor that could influence the effectiveness of immune modulation in dogs with VL and suggest that blocking IL-10R activity has the potential to be a useful approach to CVL treatment.pt_BR
dc.identifier.citationCARDOSO, J. M. de O. et al. IL-10 receptor blockade controls the in vitro infectivity of Leishmania infantum and promotes a Th1 activation in PBMC of dogs with visceral leishmaniasis. Molecular Immunology, v. 137, p. 20-27, 2021. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0161589021001899?via%3Dihub>. Acesso em: 11 out. 2022.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.molimm.2021.06.014pt_BR
dc.identifier.issn0161-5890
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/16057
dc.identifier.uri2https://www.sciencedirect.com/science/article/pii/S0161589021001899?via%3Dihubpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectCanine visceral leishmaniasispt_BR
dc.subjectAnti-canine IL-10 receptor-blocking monoclonal antibodypt_BR
dc.subjectImmunotherapypt_BR
dc.titleIL-10 receptor blockade controls the in vitro infectivity of Leishmania infantum and promotes a Th1 activation in PBMC of dogs with visceral leishmaniasis.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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