Differential parasitological, molecular, and serological detection of Trypanosoma cruzi I, II, and IV in blood of experimentally infected mice.

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dc.contributor.authorTeston, Ana Paula Margioto
dc.contributor.authorAbreu, Ana Paula de
dc.contributor.authorGruendling, Ana Paula
dc.contributor.authorBahia, Maria Terezinha
dc.contributor.authorGomes, Mônica Lúcia
dc.contributor.authorAraújo, Silvana Marques de
dc.contributor.authorToledo, Max Jean de Ornelas
dc.date.accessioned2017-12-20T15:04:13Z
dc.date.available2017-12-20T15:04:13Z
dc.date.issued2016
dc.description.abstractTrypanosoma cruzi is the etiological agent of American trypanosomiasis (Chagas' disease), which affects 6 e7 million people worldwide, mainly in Latin America. It presents great genetic and biological variability that plays an important role in the clinical and epidemiological features of the disease. Our working hypothesis is that the genetic diversity of T. cruzi has an important impact on detection of the parasite using diagnostic techniques. The present study evaluated the diagnostic performance of parasitological, molecular, and serological techniques for detecting 27 strains of T. cruzi that belonged to discrete typing units (DTUs) TcI (11 strains), TcII (four strains), and TcIV (12 strains) that were obtained from different hosts in the states of Amazonas and Paran a, Brazil. Blood samples were taken from experimentally infected mice and analyzed by fresh blood examination, hemoculture in Liver Infusion Tryptose (LIT) medium, polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA). Polymerase chain reaction presented the best detection of TcI, with 80.4% positivity. For all of the detection methods, the animals that were inoculated with TcII presented the highest positivity rates (94.1e100%). ELISA that was performed 7 months after inoculation presented a higher detection ability (95.4%) for TcIV. Intra-DTU comparisons showed that the reproducibility of the majority of the results that were obtained with the different methods was weak for TcI and good for TcII and TcIV. Our data indicate that the detection capability of different techniques varies with the DTUs of the parasites in mammalian blood. The implications of these findings with regard to the diagnosis of human T. cruzi infection are discussed.pt_BR
dc.identifier.citationTESTON, A. P. M. et al. Differential parasitological, molecular, and serological detection of Trypanosoma cruzi I, II, and IV in blood of experimentally infected mice. Experimental Parasitology, v. 166, p. 44-50, 2016. Disponível em: <http://www.sciencedirect.com/science/article/pii/S001448941630039X?via%3Dihub>. Acesso em: 15 set. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.exppara.2016.03.013
dc.identifier.issn0014-4894
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/9223
dc.identifier.uri2http://www.sciencedirect.com/science/article/pii/S001448941630039X?via%3Dihubpt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectHemoculturept_BR
dc.titleDifferential parasitological, molecular, and serological detection of Trypanosoma cruzi I, II, and IV in blood of experimentally infected mice.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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