Characterization and mRNA expression analysis of PI31, an endogenous proteasome inhibitor from Schistosoma mansoni.
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2010
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Abstract
The proline-rich inhibitor of 31 kDa (PI31) is
highly conserved through metazoan evolution, and its
activity in the proteasome inhibition is well-established
although the precise mechanism of inhibition is unclear.
The coding DNA sequence of Schistosoma mansoni PI31
(SmPI31) was cloned, and the recombinant protein was
expressed in bacterial system. The correct amino acid
sequence was confirmed by mass spectrometry and circular
dichroism suggests that SmPI31 contains both α-helix and
non-structured regions. Inhibition assays, using the SucLeu-Leu-Val-Tyr-4-MCA
substrate for proteasome degradation,
showed that the S. mansoni proteasome may be
regulated by the inhibitory activity of SmPI31. A gene
expression assay using qRT-PCR at various stages during
the S. mansoni life cycle has shown that SmPI31 transcripts
are expressed in all studied stages, suggesting that PI31
plays an important role during the developmental processes
of the parasite. In this study first evidence is presented that
PI31 has a conserved structure and plays a role as
proteasome inhibitor in adult worms and it is expressed
through life cycle.
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MACHADO, C. B. et al. Characterization and mRNA expression analysis of PI31, an endogenous proteasome inhibitor from Schistosoma mansoni. Parasitology Research, v. 107, p. 1163-1171, 2010. Disponível em: <https://link.springer.com/article/10.1007%2Fs00436-010-1984-x>. Acesso em: 23 fev. 2017.