Germ-free mice produce high levels of interferon-gamma in response to infection with Leishmania major but fail to heal lesions.

Simple item page
dc.contributor.authorOliveira, Marcia Rosa de
dc.contributor.authorTafuri, Wagner Luiz
dc.contributor.authorAfonso, Luís Carlos Crocco
dc.contributor.authorOliveira, Milton Adriano Pelli de
dc.contributor.authorNicoli, Jacques Robert
dc.contributor.authorVieira, Etel Rocha
dc.contributor.authorScott, Phillip
dc.contributor.authorMelo, Maria Norma
dc.contributor.authorVieira, Leda Quercia
dc.date.accessioned2017-06-13T15:57:39Z
dc.date.available2017-06-13T15:57:39Z
dc.date.issued2005
dc.description.abstractIn order to investigate the importance of the host microbiota on differentiation of T cell subsets in response to infection, Swiss/NIH germ-free mice and conventional (microbiota-bearing) mice were infected with Leishmania major, and lesion development, parasite loads, and cytokine production were assessed. Germ-free mice failed to heal lesions and presented a higher number of parasites at the site of infection than their conventional counterparts. In addition, histopathological analysis indicated a higher density of parasitized macrophages in lesions from germ-free mice than in conventional mice. The initial production of interleukin (IL)-12 and interferon-gamma (IFN-c) in germ-free mice was comparable to the conventional controls. Also, germ-free mice produced elevated levels of IFN-c and lower levels of IL-4 throughout the course of infection, suggesting the development of a Th1 response. Macrophages from germ-free mice exposed to IFN-c and infected with amastigotes in vitro were not as efficient at killing parasites as macrophages from conventional animals. These observations indicate that the microbiota is not essential for the development of Th1 immune responses, but seems to be important for macrophage activation.pt_BR
dc.identifier.citationOLIVEIRA, M. R. de. Germ-free mice produce high levels of interferon-gamma in response to infection with Leishmania major but fail to heal lesions. Parasitology, Reino Unido, v. 131, p. 477-488, 2005. Disponível em: <https://goo.gl/IaLWv7>. Acesso em: 19 fev. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.1017/S0031182005008073
dc.identifier.issn1469-8161
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/7961
dc.identifier.uri2https://goo.gl/IaLWv7pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectLeishmaniasispt_BR
dc.subjectMicrobiotapt_BR
dc.subjectGnotobioticpt_BR
dc.subjectGnotobiologypt_BR
dc.titleGerm-free mice produce high levels of interferon-gamma in response to infection with Leishmania major but fail to heal lesions.pt_BR
dc.typeArtigo publicado em periodicopt_BR
Files
Collections
DECBI - Artigos publicados em periódicos