Association of electroanalytical and spectrophotometric methods to evaluate the antioxidant activity of isobenzofuranone in primary cultures of hippocampal neurons.

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dc.contributor.authorTeixeira, Aniely dos Reis
dc.contributor.authorTeixeira, Róbson Ricardo
dc.contributor.authorRibeiro, Iara Mariana Léllis
dc.contributor.authorPereira, Wagner Luiz
dc.contributor.authorManhabosco, Taíse Matte
dc.contributor.authorBrito, Ana Carolina Ferreira de
dc.contributor.authorOliveira, Laser Antônio Machado de
dc.contributor.authorNogueira, Katiane de Oliveira Pinto Coelho
dc.date.accessioned2021-11-22T11:55:30Z
dc.date.available2021-11-22T11:55:30Z
dc.date.issued2020pt_BR
dc.description.abstractThe isobenzofuran-1(3H)-ones (phthalides) exhibit various biological activities, including antioxidant activity on reactive oxygen species (ROS). An excess of ROS that cannot be naturally contained by cellular enzymatic systems is called redox imbalance, which damage cell membranes, proteins, and DNA, thereby possibly triggering neuronal death in several neurodegenerative diseases. Considering our ongoing efforts to find useful compounds to control redox imbalance, herein we evaluated the antioxidant activity of two phtalides (compounds 3 and 4), using primary cultures of hippocampal neurons. Spectrophotometric assays showed that compound 3 significantly reduced (p ≤ 0.05) ROS levels and lipid peroxidation compared to the control treatment, while compound 4 was unable at any of the tested concentrations. Despite their structural similarity, these compounds behave differently in the intracellular environment, which was reliably corroborated by the determination of oxidation potentials via cyclic voltammetry. It was demonstrated that compound 3 presents a lower oxidation potential. The combination of the mentioned methods allowed us to find a strong correlation between the chemical structure of compounds and their biological effects. Taking together, the results indicate that compound 3 presents desirable characteristics to act as a candidate pharmacological agent for use in the prevention and treatment of neurodegenerative diseases.pt_BR
dc.identifier.citationTEIXEIRA, A. dos R. et al. Association of electroanalytical and spectrophotometric methods to evaluate the antioxidant activity of isobenzofuranone in primary cultures of hippocampal neurons. Toxicology in Vitro, v. 68, p. 104970, 2020. Disponível em: <https://www.sciencedirect.com/science/article/abs/pii/S0887233320305208>. Acesso em: 10 jun. 2021.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.tiv.2020.104970pt_BR
dc.identifier.issn0887-2333
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/13985
dc.identifier.uri2https://www.sciencedirect.com/science/article/abs/pii/S0887233320305208pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectCyclic voltammetrypt_BR
dc.subjectFree radicalspt_BR
dc.titleAssociation of electroanalytical and spectrophotometric methods to evaluate the antioxidant activity of isobenzofuranone in primary cultures of hippocampal neurons.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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