Analysis using canine peripheral blood for establishing in vitro conditions for monocyte differentiation into macrophages for Leishmania chagasi infection and T-cell subset purification.

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dc.contributor.authorViana, Kelvinson Fernandes
dc.contributor.authorSoares, Rodrigo Dian de Oliveira Aguiar
dc.contributor.authorRoatt, Bruno Mendes
dc.contributor.authorResende, Lucilene Aparecida
dc.contributor.authorLemos, Denise da Silveira
dc.contributor.authorOliveira, Rodrigo Corrêa de
dc.contributor.authorMartins Filho, Olindo Assis
dc.contributor.authorMoura, Sandra Aparecida Lima de
dc.contributor.authorZanini, Marcos Santos
dc.contributor.authorAraújo, Márcio Sobreira Silva
dc.contributor.authorReis, Alexandre Barbosa
dc.contributor.authorGiunchetti, Rodolfo Cordeiro
dc.date.accessioned2015-01-22T14:53:07Z
dc.date.available2015-01-22T14:53:07Z
dc.date.issued2013
dc.description.abstractCanine visceral leishmaniasis (CVL) is a parasitic disease endemic in many countries, anddogs present as the major natural reservoir of the parasite, Leishmania chagasi (syn. L.infantum). Biomarkers in the canine immune system is an important technique in thecourse of developing vaccines and treatment strategies against CVL. New methodologiesfor studying the immune response of dogs during Leishmania infection and after receivingvaccines and treatments against CVL would be useful. In this context, we used peripheralblood mononuclear cells (PBMCs) from healthy dogs to evaluate procedures related to (i)establishment of in vitro conditions of monocytes differentiated into macrophages infectedwith L. chagasi and (ii) purification procedures of T-cell subsets (CD4+and CD8+) usingmicrobeads. Our data demonstrated that after 5 days of differentiation, macrophages wereable to induce significant phagocytic and microbicidal activity after L. chagasi infectionand also showed increased frequency of parasitism and a higher parasite load. Although N-acetyl- _-d-glucosaminidase (NAG) levels presented similar levels of macrophage cultureand L. chagasi infection, a progressive decrease in myeloperoxidase (MPO) levels was ahallmark over 5 days of culture. High purity levels (>90%) of CD4 and CD8 T cells wereobtained on a magnetic separation column. We concluded that monocytes differentiatedinto macrophages at 5 days and displayed an intermediate frequency of parasitism andparasite load 72 h after L. chagasi infection. Furthermore, the purification system usingcanine T-lymphocyte subsets obtained after 5 days of monocyte differentiation provedefficient for CD4 or CD8 T-cell purification (≥90%). The in vitro analysis using L. chagasi-infected macrophages and purified T cells presented a prospective methodology that couldbe incorporated in CVL vaccine and treatment studies that aim to analyze the microbicidalpotential induced by specific CD4+and/or CD8+T cells.pt_BR
dc.identifier.citationVIANA, K. F. et al. Analysis using canine peripheral blood for establishing in vitro conditions for monocyte differentiation into macrophages for Leishmania chagasi infection and T-cell subset purification. Veterinary Parasitology, v. 198, p. 62-71, 2013. Disponível em: <http://www.sciencedirect.com/science/article/pii/S0304401713004779>. Acesso em: 12 ago. 2014.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.vetpar.2013.08.014
dc.identifier.issn0304-4017
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4327
dc.language.isoen_USpt_BR
dc.rights.licenseO periódico Veterinary Parasitology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3440300468469.pt_BR
dc.subjectLeishmania chagasipt_BR
dc.subjectLeishmaniasis visceral caninept_BR
dc.subjectMcrobicidal analysispt_BR
dc.subjectMacrophagept_BR
dc.titleAnalysis using canine peripheral blood for establishing in vitro conditions for monocyte differentiation into macrophages for Leishmania chagasi infection and T-cell subset purification.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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