Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.

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dc.contributor.authorPrado, Marco Antônio Maximo
dc.contributor.authorSantos, T. Moraes
dc.contributor.authorFreitas, Renata Nascimento de
dc.contributor.authorSilva, Marco Aurélio Romano
dc.contributor.authorGomez, Marcus Vinicius
dc.date.accessioned2015-04-01T17:30:11Z
dc.date.available2015-04-01T17:30:11Z
dc.date.issued1990
dc.description.abstractA chemiluminescent method has been used recently for the determination of acetylcholine with limitations such as the presence of a cholinesterase inhibitor in the incubation medium, which is indispensable for the study of acetylcholine release by various agents. A modified procedure is presented in which the cholinesterase inhibitor eserine (physostigmine) is extracted from the medium. The results showed complete recovery when labelled acetylcholine was used. This modified procedure was used to determine the release of acetylcholine evoked by tityustoxin and ouabain. The results were comparable to those obtained by bioassay using a strip of guinea pig ileum.pt_BR
dc.identifier.citationPRADO, M. A. M. et al. Choline oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices. Journal of Neuroscience Methods, v. 31, p. 193-196, 1990. Disponível em: <https://www.sciencedirect.com/science/article/pii/0165027090901102>. Acesso em: 08 nov. 2014.pt_BR
dc.identifier.doihttps://doi.org/10.1016/0165-0270(90)90110-2
dc.identifier.issn0165-0270
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4916
dc.language.isoen_USpt_BR
dc.rights.licenseO periódico Journal of Neuroscience Methods concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3556580424364.pt_BR
dc.subjectAcetylcholine assaypt_BR
dc.subjectChemiluminescent procedurept_BR
dc.subjectNeurotransmitter releasept_BR
dc.titleCholine oxidase chemiluminéscent assay, after removal of eserine from medium, of acetylcholine released in vitro from brain slices.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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