Unraveling the secrets of a double-life fungus by genomics : ophiocordyceps australis CCMB661 displays molecular machinery for both parasitic and endophytic lifestyles.

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dc.contributor.authorMenezes, Thaís Almeida de
dc.contributor.authorAburjaile, Flávia Figueira
dc.contributor.authorPeixoto, Gabriel Quintanilha
dc.contributor.authorTomé, Luiz Marcelo Ribeiro
dc.contributor.authorFonseca, Paula Luize Camargos
dc.contributor.authorPereira, Thairine Mendes
dc.contributor.authorAraújo, Daniel Silva
dc.contributor.authorMelo, Tarcisio Silva
dc.contributor.authorKato, Rodrigo Bentes
dc.contributor.authorDelabie, Jacques Hubert Charles
dc.contributor.authorRibeiro, Sérvio Pontes
dc.contributor.authorBrenig, Bertram
dc.contributor.authorAzevedo, Vasco Ariston de Carvalho
dc.contributor.authorSantos, Elisandro Ricardo Drechsler
dc.contributor.authorAndrade, Bruno Silva
dc.contributor.authorGóes Neto, Aristóteles
dc.date.accessioned2023-08-16T19:39:32Z
dc.date.available2023-08-16T19:39:32Z
dc.date.issued2023pt_BR
dc.description.abstractOphiocordyceps australis (Ascomycota, Hypocreales, Ophiocordycipitaceae) is a classic entomopathogenic fungus that parasitizes ants (Hymenoptera, Ponerinae, Ponerini). Nonetheless, according to our results, this fungal species also exhibits a complete set of genes coding for plant cell wall degrading Carbohydrate-Active enZymes (CAZymes), enabling a full endophytic stage and, consequently, its dual ability to both parasitize insects and live inside plant tissue. The main objective of our study was the sequencing and full characterization of the genome of the fungal strain of O. australis (CCMB661) and its predicted secretome. The assembled genome had a total length of 30.31 Mb, N50 of 92.624 bp, GC content of 46.36%, and 8,043 protein-coding genes, 175 of which encoded CAZymes. In addition, the primary genes encoding proteins and critical enzymes during the infection process and those responsible for the host–pathogen interaction have been identified, including proteases (Pr1, Pr4), aminopeptidases, chitinases (Cht2), adhesins, lectins, lipases, and behavioral manipulators, such as enterotoxins, Protein Tyrosine Phosphatases (PTPs), and Glycoside Hydrolases (GHs). Our findings indicate that the presence of genes coding for Mad2 and GHs in O. australis may facilitate the infection process in plants, suggesting interkingdom colonization. Furthermore, our study elucidated the pathogenicity mechanisms for this Ophiocordyceps species, which still is scarcely studied.pt_BR
dc.identifier.citationMENEZES, T. A. de et al. Unraveling the secrets of a double-life fungus by genomics: ophiocordyceps australis CCMB661 displays molecular machinery for both parasitic and endophytic lifestyles. Journal of Fungi, v. 9, n. 1, artigo 110, jan. 2023. Disponível em: <https://www.mdpi.com/2309-608X/9/1/110>. Acesso em: 15 mar. 2023.pt_BR
dc.identifier.doihttps://doi.org/10.3390/jof9010110pt_BR
dc.identifier.issn2309-608X
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/17212
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Fonte: PDF do artigo.pt_BR
dc.subjectOphiocordycipitaceaept_BR
dc.subjectEntomopathogenicitypt_BR
dc.subjectFungal genomicspt_BR
dc.subjectInsect-fungus symbiosispt_BR
dc.subjectZombie-ant funguspt_BR
dc.titleUnraveling the secrets of a double-life fungus by genomics : ophiocordyceps australis CCMB661 displays molecular machinery for both parasitic and endophytic lifestyles.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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