Copper(I)−Phosphine polypyridyl complexes : synthesis, characterization, DNA/HSA binding study, and antiproliferative activity.
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2017
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Abstract
A series of copper(I)−phosphine polypyridyl
complexes have been investigated as potential antitumor agents.
The complexes [Cu(PPh3)2dpq]NO3 (2), [Cu(PPh3)2dppz]NO3
(3), [Cu(PPh3)2dppa]NO3 (4), and [Cu(PPh3)2dppme]NO3 (5)
were synthesized by the reaction of [Cu(PPh3)2NO3] with the
respective planar ligand under mild conditions. These copper
complexes were fully characterized by elemental analysis, molar
conductivity, FAB-MS, and NMR, UV−vis, and IR spectroscopies.
Interactions between these copper(I)−phosphine polypyridyl
complexes and DNA have been investigated using various
spectroscopic techniques and analytical methods, such as UV−vis
titrations, thermal denaturation, circular dichroism, viscosity
measurements, gel electrophoresis, and competitive fluorescent intercalator displacement assays. The results of our studies
suggest that these copper(I) complexes interact with DNA in an intercalative way. Furthermore, their high protein binding
affinities toward human serum albumin were determined by fluorescence studies. Additionally, cytotoxicity analyses of all
complexes against several tumor cell lines (human breast, MCF-7; human lung, A549; and human prostate, DU-145) and nontumor
cell lines (Chinese hamster lung, V79-4; and human lung, MRC-5) were performed. The results revealed that copper(I)−
phosphine polypyridyl complexes are more cytotoxic than the corresponding planar ligand and also showed to be more active
than cisplatin. A good correlation was observed between the cytostatic activity and lipophilicity of the copper(I) complexes
studied here.
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VILARREAL, W. et al. Copper(I)−Phosphine polypyridyl complexes: synthesis, characterization, DNA/HSA binding study, and antiproliferative activity. Inorganic Chemistry, v. 56, p. 3781-3793, 2017. Disponível em: <http://pubs.acs.org/doi/pdf/10.1021/acs.inorgchem.6b02419>. Acesso em: 15 set. 2017.