Establishment of a microplate assay for flow cytometric assessment and it is use for the evaluation of age-related phenotypic changes in canine whole blood leukocytes.

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dc.contributor.authorReis, Alexandre Barbosa
dc.contributor.authorCarneiro, Cláudia Martins
dc.contributor.authorCarvalho, Maria das Graças
dc.contributor.authorCarvalho, Andréa Teixeira de
dc.contributor.authorGiunchetti, Rodolfo Cordeiro
dc.contributor.authorMayrink, Wilson
dc.contributor.authorGenaro, Odair
dc.contributor.authorOliveira, Rodrigo Corrêa de
dc.contributor.authorMartins Filho, Olindo Assis
dc.date.accessioned2012-07-10T17:52:02Z
dc.date.available2012-07-10T17:52:02Z
dc.date.issued2005
dc.description.abstractThe effectiveness of flow cytometric assays for canine use is still requiring standardization. Despite several studies using purified mononuclear cells, no methodology or reference ranges are available for immunophenotyping of whole blood leukocytes (WBL). Fresh and pre-fixed WBL were used to identify cell-subsets, (Thy-1 + /CD5 + /CD4 + /CD8 + /CD21 + and CD14 + ) and measure MHC-II, CD45RA/CD45RB expression. We described here an efficient method for fast quantification of canine-WBL, using pre-fix in a microplate assay, which allows long-term sample storage prior to phenotyping. Decreased percentage of CD5 + -T-cells within the lymphocyte-gate and increased percentage of CD21 + -B-cells were observed in young animals, which led to higher T/B cell ratios in middle-aged dogs. Lower numerical counts of Thy-1 + , CD4 + , CD8 + and CD21 + lymphocyte were observed when compared to young animals. In addition, we identified an age-related decline of MHC-II/ CD45RA expression by lymphocytes. We proposed an improved method for phenotyping of canine peripheral blood mononuclear cells (PBMC) that has significant use for researchers and veterinary clinicians. The hematological changes of senescence previously identified on PBMC could be adequately reproduced on features identified by whole blood. Furthermore, this study supplies normal range references as baseline standards for clinical purposes, besides specific immunological parameters to monitor canine aging process.pt_BR
dc.identifier.citationREIS, A. B. et al. Establishment of a microplate assay for flow cytometric assessment and it is use for the evaluation of age-related phenotypic changes in canine whole blood leukocytes. Veterinary Immunology and Immunopathology, v. 103, n. 3-4, p. 173-185, fev. 2005. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0165242704002569>. Acesso em: 10 jul. 2012.pt_BR
dc.identifier.issn01652427
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/1064
dc.language.isoen_USpt_BR
dc.rights.licenseO periódico Veterinary Immunology and Immunopa concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3282590863972.
dc.subjectFlow cytometrypt_BR
dc.subjectCaninept_BR
dc.subjectPeripheral bloodpt_BR
dc.subjectLymphocyte subsetspt_BR
dc.subjectAngingpt_BR
dc.titleEstablishment of a microplate assay for flow cytometric assessment and it is use for the evaluation of age-related phenotypic changes in canine whole blood leukocytes.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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