The ecto-5′nucleotidase/cd73 mediates Leishmania amazonensis survival in macrophages.
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2022
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Abstract
Endogenous nucleotides produced by various group of cells under inflammatory conditions act as potential danger signals in vivo.
Extracellularly released nucleotides such as ATP are rapidly hydrolyzed to adenosine by the coordinated ectonucleotidase
activities of CD39 and CD73. Leishmania is an obligate intracellular parasite of macrophages and capable of modulating host
immune response in order to survive and multiply within host cells. In this study, the activity of CD73 induced by Leishmania
amazonensis in infected macrophages has been investigated and correlated with parasite survival and infection in vitro. For
this, the expression of CD39 and CD73, by flow cytometry, in murine peritoneal macrophages infected with metacyclic
promastigotes of L. amazonensis has been analyzed. Our results showed that L. amazonensis-infected macrophages, unlike
LPS-treated macrophages, increased CD73 expression. It was also noted that when CD73 enzymatic activity was blocked by α,
β-methyleneadenosine 5′-diphosphate sodium salt (APCP), macrophage parasitism was significantly decreased. Interestingly,
these effects were not associated with the production of TNF-α, IL-10, or nitric oxide (NO). Together, these data demonstrate
that L. amazonensis induces a regulatory phenotype in macrophages, which by activating the CD39/CD73 pathway allows
parasite survival through the action of immunomodulatory adenosine receptors.
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BAJRACHARYA, B. et al. The ecto-5′nucleotidase/cd73 mediates Leishmania amazonensis survival in macrophages. BioMed Research International, v. 2022, artigo 9928362, 2022. Disponível em: <https://www.hindawi.com/journals/bmri/2022/9928362/>. Acesso em: 11 out. 2022.