Inhibitory efects of 7-epiclusianone on glucan synthesis, acidogenicityand biofilm formation by Streptococcus mutans.
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2008
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Abstract
The aim of this study was to examine the effects of 7-epiclusianone, a new
prenylated benzophenone isolated from the plant Rheedia gardneriana, on some of
the virulence properties of Streptococcus mutans associated with biofilm development
and acidogenicity. The synthesis of glucans by glucosyltransferases B (GTF B)
and C (GTF C) was markedly reduced by 7-epiclusianone showing more than 80%
inhibition of enzymatic activity at a concentration of 100 mgmL 1. Doublereciprocal
analysis (Lineweaver–Burk plots) revealed that the inhibition of GTF B
activity was noncompetitive (mixed) while GTF C was inhibited uncompetitively.
The glycolytic pH drop by S. mutans cells was also disrupted by 7-epiclusianone
without affecting the bacterial viability, an effect that can be attributed, in part, to
inhibition of F-ATPase activity (61.1 3.0% inhibition at 100 mgmL 1). Furthermore,
topical applications (1-min exposure, twice daily) of 7-epiclusianone (at
250 mgmL 1) disrupted biofilm formation and physiology. The biomass (dryweight),
extracellular insoluble polysaccharide concentration and acidogenicity of
the biofilms were significantly reduced by the test agent (Po0.05). The data show
that 7-epiclusianone disrupts the extracellular and intracellular sugar metabolism
of S. mutans, and holds promise as a novel, naturally occurring compound to
prevent biofilm-related oral diseases.
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Keywords
7-epiclusianone, Glucosyltransferases, Glycolysis, Streptococcus mutans
Citation
MURATA, R. M. et al. Inhibitory efects of 7-epiclusianone on glucan synthesis, acidogenicityand biofilm formation by Streptococcus mutans. FEMS Microbiology Letters, v. 282, p. 174-181, 2008. Disponível em: <https://academic.oup.com/femsle/article-lookup/doi/10.1111/j.1574-6968.2008.01117.x>. Acesso em: 20 mai. 2017.