The golden mussel proteome and its response to niclosamide : uncovering rational targets for control or elimination.
No Thumbnail Available
Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The Asian invasive species Limnoperna fortunei (Dunker, 1857), known as the golden mussel, causes great economic and environmental damage due to its fixative capacity and accelerated proliferation. Molecular studies for
the control of larval and adult forms are of great economic, scientific and technological interest. Here, we first
report on the compositional analysis of the L. fortunei proteome obtained through shotgun analysis using LC-MS/
MS. Among those 2790 proteins identified, many of them related to secretory processes and membrane receptors. Our second approach consisted in exposing the mollusc to the molluscicide niclosamide to evaluate the
induced proteomic alterations. Exposure to niclosamide at 0.25 mg/L for 24 h resulted in a pronounced differential abundance of proteins when compared to those obtained when exposure was reduced to 4 h at 2.3 mg/
L. In total, 342 proteins were found differentially expressed in the responsive individuals as revealed by labelfree quantitative proteomics. Regarding the affected cell processes were: cell division and differentiation, cytoskeletal organization and compartment acidification (upregulated), and energy metabolism (downregulated).
Our findings constitute the first inventory of the expressed proteome of the golden mussel and have the potential
to contribute with a more rational proposition of molecular targets for control and monitoring of this species.
Significance: With the recent availability of transcriptomic and genomic data applied to L. fortunei the timing is
right to interrogate its putative gene repertoire using proteomic techniques. These have the potential to validate
the existence of the predicted genes, infer their relative abundance and quantify their levels as a response to
environmental stressors or various agents. Here we provided an inventory of the golden mussel proteome and
evaluated its response to the molluscicide niclosamide. The obtained results open new avenues for intervention
aimed at its control or elimination, particularly by targeting the various cellular processes that were uncovered.
Description
Keywords
Limnoperna fortunei, Proteomic analysis
Citation
SANSON, A. L. et al. The golden mussel proteome and its response to niclosamide: uncovering rational targets for control or elimination. Journal of Proteomics, v. 217, abr. 2020. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1874391920300191>. Acesso em: 10 mar. 2020.