Trypanosoma cruzi : blood parasitism kinetics and their correlation with heart parasitism intensity during long-term infection of Beagle dogs.

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dc.contributor.authorVeloso, Vanja Maria
dc.contributor.authorGuedes, Paulo Marcos da Matta
dc.contributor.authorAndrade, Isabel Mayer de
dc.contributor.authorCaldas, Ivo Santana
dc.contributor.authorMartins, Helen Rodrigues
dc.contributor.authorCarneiro, Cláudia Martins
dc.contributor.authorCoelho, George Luiz Lins Machado
dc.contributor.authorLana, Marta de
dc.contributor.authorGalvão, Lúcia Maria da Cunha
dc.contributor.authorBahia, Maria Terezinha
dc.contributor.authorChiari, Egler
dc.date.accessioned2015-11-26T18:24:34Z
dc.date.available2015-11-26T18:24:34Z
dc.date.issued2008
dc.description.abstractThe goals of the present study were to evaluate the kinetics of blood parasitism by examination of fresh blood, blood culture (BC) and PCR assays and their correlation with heart parasitism during two years of infection in Beagle dogs inoculated with the Be-78, Y and ABC Trypanosoma cruzi strains. Our results showed that the parasite or its kDNA is easily detected during the acute phase in all infected animals. On the other hand, a reduced number of positive tests were verified during the chronic phase of the infection. The frequency of positive tests was correlated with T. cruzi strain. The percentage of positive BC and blood PCR performed in samples from animals inoculated with Be-78 and ABC strains were similar and significantly larger in relation to animals infected with the Y strain. Comparison of the positivity of PCR tests performed using blood and heart tissue samples obtained two years after infection showed two different patterns associated with the inoculated T. cruzi strain: (1) high PCR positivity for both blood and tissue was observed in animals infected with Be-78 or ABC strains; (2) lower and higher PCR positivity for the blood and tissue, respectively, was detected in animals infected with Y strains. These data suggest that the sensitivity of BC and blood PCR was T. cruzi strain dependent and, in contrast, the heart tissue PCR revealed higher sensitivity regardless of the parasite stock.pt_BR
dc.identifier.citationVELOSO, V. M. et al. Trypanosoma cruzi: blood parasitism kinetics and their correlation with heart parasitism intensity during long-term infection of Beagle dogs. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 6, p. 528-534, set. 2008. Disponível em: <http://www.scielo.br/pdf/mioc/v103n6/03.pdf>. Acesso em: 21 out. 2015pt_BR
dc.identifier.doihttp://dx.doi.org/10.1590/S0074-02762008000600003
dc.identifier.issn1678-8060
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/5842
dc.language.isoen_USpt_BR
dc.rights.licenseTodo o conteúdo do periódico Memórias do Instituto Oswaldo Cruz, exceto onde identificado, está sob uma licença Creative Commons que permite copiar, distribuir e transmitir o trabalho em qualquer suporte ou formato desde que sejam citados o autor e o licenciante. Não permite o uso para fins comerciais. Fonte: Memórias do Instituto Oswaldo Cruz <http://www.scielo.br/scielo.php/script_sci_serial/pid_0074-0276/lng_pt/nrm_iso>. Acesso em: 19 ago. 2019.pt_BR
dc.subjectParasitemiapt_BR
dc.subjectBlood culturept_BR
dc.subjectBlood and tissue PCRpt_BR
dc.titleTrypanosoma cruzi : blood parasitism kinetics and their correlation with heart parasitism intensity during long-term infection of Beagle dogs.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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