Influence of incubation conditions on the specific methanogenic activity test.
No Thumbnail Available
Date
2010
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The main objective of this work was to
evaluate the influence of incubating conditions on the
specific methanogenic activity (SMA) test in order to
harmonize the test protocol. For this serum bottles
were incubated with anaerobic sludge (from UASB
reactor treating domestic sewage) in factorial planned
experiments which assessed the influence of the
temperature, substrate concentration, food/microorganism
(F/M) ratio, presence of yeast extract in the
medium, as well as type of carbon and nutrient
solution. The results showed that the tested methane
measuring methods (volumetric with biogas characterization,
volumetric with gas wash in alkaline
solution, and manometric by using the OxiTop system)
presented a similar performance. The maintenance of a
small gaseous phase volume (e.g. 10% of the total
volume) resulted in higher SMA values; and the ideal
substrate concentration for the SMA test ranged from
0.5 to 3.0 gCOD/l since higher acetate concentration
caused sludge inhibition. The suggested temperature
for the test is 35 C and the best F/M ratio varied from
0.125 to 0.750 gCOD/gVS, and this seemed to be the
most influent parameter for the SMA test. Finally tests
performed with nutrient solution complemented by
yeast extract resulted in the highest SMA values.
Description
Keywords
Anaerobic digestion, Reactor control operation, Wastewater treatment
Citation
SOUTO, T. F. et al. Influence of incubation conditions on the specific methanogenic activity test. Biodegradation, v. 21, p. 411-424, 2010. Disponível em: <https://link.springer.com/article/10.1007%2Fs10532-009-9311-x>. Acesso em: 20 mar. 2017.