A potential link among antioxidant enzymes, histopathology, and trace elements in canine visceral leishmaniasis.
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Date
2014
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Abstract
Canine visceral leishmaniasis (CVL) is a severe and fatal systemic chronic inflammatory
disease. We investigated the alterations in, and potential associations among,
antioxidant enzymes, trace elements and histopathology in CVL. Blood and tissue
levels of Cu-Zn superoxide dismutase, catalase and glutathione peroxidase were
measured in mixed-breed dogs naturally infected with Leishmania infantum chagasi,
symptomatic (n = 19) and asymptomatic (n = 11). Serum levels of copper, iron, zinc,
selenium and nitric oxide, and plasma lipid peroxidation were measured. Histological
and morphometric analyses were conducted of lesions in liver, spleen and lymph
nodes. We found lower blood catalase and glutathione peroxidase activity to be correlated
with lower iron and selenium respectively. However, higher activity of Cu-
Zn superoxide dismutase was not correlated with the increase in copper and
decreased in zinc observed in infected animals compared to controls. Organ tissue
was characterized by lower enzyme activity in infected dogs than in controls, but
this was not correlated with trace elements. Lipid peroxidation was higher in symptomatic
than in asymptomatic and control dogs and was associated with lesions such
as chronic inflammatory reaction, congestion, haemosiderin and fibrosis. Systemic
iron deposition was observed primarily in the symptomatic dogs showing a higher
tissue parasite load. Dogs with symptomatic CVL displayed enhanced LPO and Fe
tissue deposition associated with decreased levels of antioxidant enzymes. These
results showed new points in the pathology of CVL and might open new treatment
perspectives associated with antioxidants and the role of iron in the pathogenesis of
CVL.
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Iron
Citation
SOUZA, C. C. de et al. A potential link among antioxidant enzymes, histopathology, and trace elements in canine visceral leishmaniasis. International Journal of Experimental Pathology, v. 95, p. 260-270, 2014. DisponĂvel em: <http://onlinelibrary.wiley.com/doi/10.1111/iep.12080/epdf>. Acesso em: 20 mar. 2017.