Hookworm products ameliorate dextran sodium sulfate-induced colitis in BALB/c mice.
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2011
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Abstract
Background: Several lines of evidence have shown that helminthiasis can significantly reduce disease severity in animal models of intestinal
inflammation, airway inflammation/hyperreactivity, diabetes, and multiple sclerosis. Identification and characterization of helminth-derived immunomodulatory
molecules that contribute to anticolitis effects could lead to new therapeutic approaches in inflammatory bowel diseases (IBDs)
without the need for helminth infection. We evaluated the therapeutic potential of adult human hookworm, Ancylostoma ceylanicum, crude (Aw)
and excreted/secreted (ES) products on dextran sulfate sodium (DSS)-induced colitis in BALB/c mice.
Methods: Colitis was induced by 5% DSS oral administration for 7 days. Clinical disease severity was monitored daily during concomitant intraperitoneal
treatment with helminth-derived products. Additionally, several pathways of immunological modulation induced by A. ceylanicum
products (MPO, EPO, Th1, Th2, and Th17 cytokine responses) in the inflamed intestinal microenvironment were assessed. Finally, the histopathological
profile of the colon was characterized.
Results: Hookworm products are able to modulate the potent proinflammatory response induced by DSS, mainly through the downregulation of
Th1 and Th17 cytokines. These proteins also reduce clinical and colonic microscopic inflammation scores as well as EPO and MPO activity.
Conclusions: Ancylostoma ceylanicum Aw and ES mediators have an important therapeutic potential in experimental colitis in mice, which
may provide a more socially acceptable form of therapy for patients with IBDs as opposed to using living worms. Our results support the urgency
of further isolation and recombinant expression of active hookworm products responsible for the beneficial effects on colitis.
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Keywords
Inflammatory bowel disease, Dextran sulfate sodium
Citation
CANÇADO, G. G. L. et al. Hookworm products ameliorate dextran sodium sulfate-induced colitis in BALB/c mice. Inflammatory Bowel Diseases, v. 17, n. 11, p. 2275-2286, nov. 2011. Disponível em: <https://academic.oup.com/ibdjournal/article/17/11/2275/4630988>. Acesso em: 10 jan. 2017.