Comparative FISH-mapping of TTAGG telomeric sequences to the chromosomes of leafcutter ants (Formicidae, Myrmicinae) : is the insect canonical sequence conserved?

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dc.contributor.authorCastro, Carini Picardi Morais de
dc.contributor.authorCardoso, Danon Clemes
dc.contributor.authorMicolino, Ricardo
dc.contributor.authorCristiano, Maykon Passos
dc.date.accessioned2021-09-09T21:20:29Z
dc.date.available2021-09-09T21:20:29Z
dc.date.issued2020pt_BR
dc.description.abstractTelomeric sequences are conserved across species. The most common sequence reported among insects is (TTAGG)n , but its universal occurrence is not a consensus because other canonical motifs have been reported. In the present study, we used fluorescence in situ hybridization (FISH) using telomeric probes with (TTAGG)6 repeats to describe the telomere composition of leafcutter ants. We performed the molecular cytogenetic characterization of six Acromyrmex Mayr, 1865 and one Atta Fabricius, 1804 species (Acromyrmex ambiguus (Emery, 1888), Ac. crassispinus (Forel, 1909), Ac. lundii (Guérin-Mèneville, 1838), Ac. nigrosetosus (Forel, 1908), Ac. rugosus (Smith, 1858), Ac. subterraneus subterraneus (Forel, 1893), and Atta sexdens (Linnaeus, 1758)) and described it using a karyomorphometric approach on their chromosomes. The diploid chromosome number 2n = 38 was found in all Acromyrmex species, and the karyotypic formulas were as follows: Ac. ambiguus 2K = 14M + 12SM + 8ST + 4A, Ac. crassispinus 2K = 12M + 20SM + 4ST + 2A, Ac. lundii 2K = 10M + 14SM + 10ST + 4A, Ac. nigrosetosus 2K = 12M + 14SM + 10ST + 2A, and Ac. subterraneus subterraneus 2K = 14M + 18SM + 4ST + 2A. The exact karyotypic formula was not established for Ac. rugosus. FISH analyses revealed the telomeric regions in all the chromosomes of the species studied in the present work were marked by the (TTAGG)6 sequence. These results reinforce the premise that Formicidae presents high homology between their genera for the presence of the canonical sequence (TTAGG)n.pt_BR
dc.identifier.citationCASTRO, C. P. M. de. et al. Comparative FISH-mapping of TTAGG telomeric sequences to the chromosomes of leafcutter ants (Formicidae, Myrmicinae): is the insect canonical sequence conserved?. Comparative Cytogenetics, v. 14, p. 369-385, ago. 2020. Disponível em: <https://compcytogen.pensoft.net/article/52726/>. Acesso em: 12 maio 2021.pt_BR
dc.identifier.doihttps://doi.org/10.3897/CompCytogen.v14i3.52726pt_BR
dc.identifier.issn1993-078X
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/13687
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: o PDF do artigo.pt_BR
dc.subjectEvolutionpt_BR
dc.subjectFISH - fluorescence in situ hybridizationpt_BR
dc.subjectLeafcutter antspt_BR
dc.subjectTelomerept_BR
dc.titleComparative FISH-mapping of TTAGG telomeric sequences to the chromosomes of leafcutter ants (Formicidae, Myrmicinae) : is the insect canonical sequence conserved?pt_BR
dc.typeArtigo publicado em periodicopt_BR
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