Identification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the Xanthomonadaceae.

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dc.contributor.authorAssis, Renata de Almeida Barbosa
dc.contributor.authorPolloni, Lorraine Cristina
dc.contributor.authorPatané, José Salvatore Leister
dc.contributor.authorThakur, Shalabh
dc.contributor.authorFelestrino, Érica Barbosa
dc.contributor.authorDiaz Caballero, Julio
dc.contributor.authorDigiampietri, Luciano Antonio
dc.contributor.authorGoulart Filho, Luiz Ricardo
dc.contributor.authorAlmeida Junior, Nalvo Franco de
dc.contributor.authorNascimento, Rafael
dc.contributor.authorDandekar, Abhaya M.
dc.contributor.authorZaini, Paulo Adriano
dc.contributor.authorSetubal, João Carlos
dc.contributor.authorGuttman, David S.
dc.contributor.authorMoreira, Leandro Marcio
dc.date.accessioned2019-04-08T18:24:20Z
dc.date.available2019-04-08T18:24:20Z
dc.date.issued2017
dc.description.abstractThe Xanthomonadaceae family consists of species of non-pathogenic and pathogenic γ-proteobacteria that infect different hosts, including humans and plants. In this study, we performed a comparative analysis using 69 fully sequenced genomes belonging to this family, with a focus on identifying proteins enriched in phytopathogens that could explain the lifestyle and the ability to infect plants. Using a computational approach, we identified seven phytopathogen-enriched protein families putatively secreted by type II secretory system: PheA (CM-sec), LipA/LesA, VirK, and four families involved in N-glycan degradation, NixE, NixF, NixL, and FucA1. In silico and phylogenetic analyses of these protein families revealed they all have orthologs in other phytopathogenic or symbiotic bacteria, and are involved in the modulation and evasion of the immune system. As a proof of concept, we performed a biochemical characterization of LipA from Xac306 and verified that the mutant strain lost most of its lipase and esterase activities and displayed reduced virulence in citrus. Since this study includes closely related organisms with distinct lifestyles and highlights proteins directly related to adaptation inside plant tissues, novel approaches might use these proteins as biotechnological targets for disease control, and contribute to our understanding of the coevolution of plant-associated bacteria.pt_BR
dc.identifier.citationASSIS, R. de A. B. et al. Identification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the Xanthomonadaceae. Scientific Reports, v. 7, p. 1-17, 2017. Disponível em: <https://www.nature.com/articles/s41598-017-16325-1>. Acesso em: 22 fev. 2019.pt_BR
dc.identifier.issn20452322
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/10970
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Fonte: o próprio artigopt_BR
dc.titleIdentification and analysis of seven effector protein families with different adaptive and evolutionary histories in plant-associated members of the Xanthomonadaceae.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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