Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a.
No Thumbnail Available
Date
2018
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
The methods currently available for genotype-specific diagnosis of T. cruzi infection still
present relevant limitations, especially to identify mixed infection. In the present investigation,
we have evaluated the performance of Chagas-Flow ATE-IgG2a test for early and late
differential diagnosis of single and dual genotype-specific T. cruzi infections. Serum samples
from Swiss mice at early and late stages of T. cruzi infection were assayed in parallel
batches for genotype-specific diagnosis of single (TcI, TcVI or TcII) and dual (TcI+TcVI,
TcVI+TcII or TcII+TcI) infections. The intrinsic reactivity to TcI, TcVI and TcII target antigens,
including amastigote (AI/AVI/AII), trypomastigote-(TI/TVI/TII) and epimastigote (EI/
EVI/EII), at specific reverse of serum dilutions (500 to 64,000), was employed to provide reliable
decision-trees for ªearlyº vs ªlateº, ªsingle vs ªdualº and ªgenotype-specificº serology.
The results demonstrated that selective set of attributes ªEII 500/EI 2,000/AII 500º were
able to provide high-quality accuracy (81%) to segregate early and late stages of T. cruzi
infection. The sets ªTI 2,000/AI 1,000/EII 1,000º and ªTI 8,000/AII 32,000º presented expressive
scores to discriminate single from dual T. cruzi infections at early (85%) and late stages
(84%), respectively. Moreover, the attributes ªTI 4,000/TVI 500/TII 1,000º, ªTI 16,000/EI
2,000/EII 2,000/AI 500/TVI 500º showed good performance for genotype-specific diagnosis
at early stage of single (72%) and dual (80%) T. cruzi infections, respectively. In addition,
the attributes ªTI 4,000/AII 1,000/EVI 1,000º, ªTI 64,000/AVI 500/AI 2,000/AII 1,000/EII
4,000º showed moderate performance for genotype-specific diagnosis at late stage of
single (69%) and dual (76%) T. cruzi infections, respectively. The sets of decision-trees
were assembled to construct a sequential algorithm with expressive accuracy (81%) for serological diagnosis of T. cruzi infection. These findings engender new perspectives for the
application of Chagas-Flow ATE-IgG2a method for genotype-specific diagnosis in humans,
with relevant contributions for epidemiological surveys as well as clinical and post-therapeutic
monitoring of Chagas disease.
Description
Keywords
Citation
DINIZ, G. D. et al. Accomplishing the genotype-specific serodiagnosis of single and dual Trypanosoma cruzi infections by flow cytometry Chagas- Flow ATE-IgG2a. PLoS Neglected Tropical Diseases, v. 12, p. 1-23, 2018. Disponível em: <http://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0006140&type=printable>. Acesso em: 05 abr. 2018.