Cytogenetic analysis and chromosomal mapping of repetitive DNA in Melipona species (Hymenoptera, Meliponini).

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dc.contributor.authorTravenzoli, Natália Martins
dc.contributor.authorLima, Bárbara Soares Amoroso
dc.contributor.authorCardoso, Danon Clemes
dc.contributor.authorSantos, Jorge Abdala Dergam dos
dc.contributor.authorSalomão, Tânia Maria Fernandes
dc.contributor.authorLopes, Denilce Meneses
dc.date.accessioned2020-05-21T15:02:55Z
dc.date.available2020-05-21T15:02:55Z
dc.date.issued2019
dc.description.abstractStingless bees of the genus Melipona are subdivided into 4 subgenera called Eomelipona, Melikerria, Melipona sensu stricto, and Michmelia according to species morphology. Cytogenetically, the species of the genus Melipona show variation in the amount and distribution of heterochromatin along their chromosomes and can be separated into 2 groups: the first with low content of heterochromatin and the second with high content of heterochromatin. These heterochromatin patterns and the number of chromosomes are characteristics exclusive to Melipona karyotypes that distinguish them from the other genera of the Meliponini. To better understand the karyotype organization in Melipona and the relationship among the subgenera, we mapped repetitive sequences and analyzed previously reported cytogenetic data with the aim to identify cytogenetic markers to be used for investigating the phylogenetic relationships and chromosome evolution in the genus. In general, Melipona species have 2n = 18 chromosomes, and the species of each subgenus share the same characteristics in relation to heterochromatin regions, DAPI/CMA3 fluorophores, and the number and distribution of 18S rDNA sites. Microsatellites were observed only in euchromatin regions, whereas the (TTAGG)6 repeats were found at telomeric sites in both groups. Our data indicate that in addition to the chromosome number, the karyotypes in Melipona could be separated into 2 groups that are characterized by conserved cytogenetic features and patterns that generally are shared by species within each subgenus, which may reflect evolutionary constraints. Our results agree with the morphological separation of the Melipona into 4 subgenera, suggesting that they must be independent evolutionary lineages.pt_BR
dc.identifier.citationTRAVENZOLI, N. M. et al. Cytogenetic analysis and chromosomal mapping of repetitive DNA in Melipona species (Hymenoptera, Meliponini). Cytogenetic and Genome Research, v. 158, n. 4, p. 213–224, set. 2019. Disponível em: <https://www.karger.com/Article/FullText/501754>. Acesso em: 10 mar. 2020.pt_BR
dc.identifier.doihttps://doi.org/10.1159/000501754pt_BR
dc.identifier.issn1424-859X
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/12221
dc.identifier.uri2https://www.karger.com/Article/FullText/501754pt_BR
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.subjectFluorescence in situ hybridizationpt_BR
dc.subjectEuchromatinpt_BR
dc.subjectHeterochromatinpt_BR
dc.subjectMolecular cytogeneticspt_BR
dc.subjectNucleotide constitutionpt_BR
dc.titleCytogenetic analysis and chromosomal mapping of repetitive DNA in Melipona species (Hymenoptera, Meliponini).pt_BR
dc.typeArtigo publicado em periodicopt_BR
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