A secreted chorismate mutase from Xanthomonas arboricola pv. juglandis attenuates virulence and walnut blight symptoms.

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dc.contributor.authorAssis, Renata de Almeida Barbosa
dc.contributor.authorSagawa, Cintia Helena Duarte
dc.contributor.authorZaini, Paulo Adriano
dc.contributor.authorSaxe, Houston J.
dc.contributor.authorWilmarth, Phillip A.
dc.contributor.authorPhinney, Brett S.
dc.contributor.authorSalemi, Michelle
dc.contributor.authorMoreira, Leandro Marcio
dc.contributor.authorDandekar, Abhaya M.
dc.date.accessioned2023-05-03T18:48:44Z
dc.date.available2023-05-03T18:48:44Z
dc.date.issued2021pt_BR
dc.description.abstractWalnut blight is a significant above-ground disease of walnuts caused by Xanthomonas arboricola pv. juglandis (Xaj). The secreted form of chorismate mutase (CM), a key enzyme of the shikimate pathway regulating plant immunity, is highly conserved between plant-associated beta and gamma proteobacteria including phytopathogens belonging to the Xanthomonadaceae family. To define its role in walnut blight disease, a dysfunctional mutant of chorismate mutase was created in a copper resistant strain Xaj417 (XajCM). Infections of immature walnut Juglans regia (Jr) fruit with XajCM were hypervirulent compared with infections with the wildtype Xaj417 strain. The in vitro growth rate, size and cellular morphology were similar between the wild-type and XajCM mutant strains, however the quantification of bacterial cells by dPCR within walnut hull tissues showed a 27% increase in XajCM seven days post-infection. To define the mechanism of hypervirulence, proteome analysis was conducted to compare walnut hull tissues inoculated with the wild type to those inoculated with the XajCM mutant strain. Proteome analysis revealed 3296 Jr proteins (five decreased and ten increased with FDR ≤ 0.05) and 676 Xaj417 proteins (235 increased in XajCM with FDR ≤ 0.05). Interestingly, the most abundant protein in Xaj was a polygalacturonase, while in Jr it was a polygalacturonase inhibitor. These results suggest that this secreted chorismate mutase may be an important virulence suppressor gene that regulates Xaj417 virulence response, allowing for improved bacterial survival in the plant tissues.pt_BR
dc.identifier.citationASSIS, R. de A. B. et al. A secreted chorismate mutase from Xanthomonas arboricola pv. juglandis attenuates virulence and walnut blight symptoms. International Journal of Molecular Sciences, v. 22, artigo 10374, 2021. Disponível em: <https://www.mdpi.com/1422-0067/22/19/10374>. Acesso em: 11 out. 2022.pt_BR
dc.identifier.doihttps://doi.org/10.3390/ijms221910374pt_BR
dc.identifier.issn1422-0067
dc.identifier.urihttp://www.repositorio.ufop.br/jspui/handle/123456789/16506
dc.language.isoen_USpt_BR
dc.rightsabertopt_BR
dc.rights.licenseThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). Fonte: o PDF do artigo.pt_BR
dc.subjectHypervirulencept_BR
dc.subjectJuglans regiapt_BR
dc.subjectWalnut blightpt_BR
dc.subjectProteomept_BR
dc.titleA secreted chorismate mutase from Xanthomonas arboricola pv. juglandis attenuates virulence and walnut blight symptoms.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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