Efficacy of Lychnopholide polymeric nanocapsules after oral and intravenous administration in murine experimental Chagas disease.

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dc.contributor.authorMello, Carlos Geraldo Campos de
dc.contributor.authorBranquinho, Renata Tupinambá
dc.contributor.authorOliveira, Maykon Tavares de
dc.contributor.authorMilagre, Matheus Marques
dc.contributor.authorGuimarães, Dênia Antunes Saúde
dc.contributor.authorMosqueira, Vanessa Carla Furtado
dc.contributor.authorLana, Marta de
dc.date.accessioned2017-08-30T17:31:17Z
dc.date.available2017-08-30T17:31:17Z
dc.date.issued2016
dc.description.abstractThe etiological treatment of Chagas disease remains neglected. The compounds available show several limitations, mainly during the chronic phase. Lychnopholide encapsulated in polymeric nanocapsules (LYC-NC) was efficacious in mice infected with Trypanosoma cruzi and treated by intravenous administration during the acute phase (AP). As the oral route is preferred for treatment of chronic infections, such as Chagas disease, this study evaluated the use of oral LYC-NC in the AP and also compared it with LYC-NC administered to mice by the oral and intravenous routes during the chronic phase (CP). The therapeutic efficacy was evaluated by fresh blood examination, hemoculture, PCR, and enzyme-linked immunosorbent assay (ELISA). The cure rates in the AP and CP were 62.5% and 55.6%, respectively, upon oral administration of LYC–poly(D,L-lactide)–polyethylene glycol nanocapsules (LYC-PLA-PEG-NC) and 57.0% and 30.0%, respectively, with LYC–poly- -caprolactone nanocapsules (LYC-PCL-NC). These cure rates were significantly higher than that of free LYC, which did not cure any animals. LYC-NC formulations administered orally during the AP showed cure rates similar to that of benznidazole, but only LYC-NC cured mice in the CP. Similar results were achieved with intravenous treatment during the CP. The higher cure rates obtained with LYC loaded in PLA-PEG-NC may be due to the smaller particle size of these NC and the presence of PEG, which influence tissue diffusion and the controlled release of LYC. Furthermore, PLA-PEG-NC may improve the stability of the drug in the gastrointestinal tract. This work is the first report of cure of experimental Chagas disease via oral administration during the CP. These findings represent a new and important perspective for oral treatment of Chagas disease.pt_BR
dc.identifier.citationMELLO, C. G. C. et al. Efficacy of Lychnopholide polymeric nanocapsules after oral and intravenous administration in murine experimental Chagas disease. Antimicrobial Agents and Chemotherapy, v. 60, p. 5215-5222, 2016. Disponível em: <http://aac.asm.org/content/60/9/5215.long>. Acesso em: 29 ago. 2017.pt_BR
dc.identifier.doihttps://doi.org/10.1128/AAC.00178-16
dc.identifier.issn1098-6596
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/8589
dc.identifier.uri2https://aac.asm.org/content/60/9/5215.long
dc.language.isoen_USpt_BR
dc.rightsrestritopt_BR
dc.titleEfficacy of Lychnopholide polymeric nanocapsules after oral and intravenous administration in murine experimental Chagas disease.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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