Heterologous expression in Caenorhabditis elegans as an alternative approach to functional studies in Schistosoma mansoni.

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dc.contributor.authorGava, Sandra Grossi
dc.contributor.authorScholte, Larissa Lopes Silva
dc.contributor.authorVolpini, Ângela Cristina
dc.contributor.authorOliveira, Riva de Paula
dc.contributor.authorOliveira, Guilherme Corrêa de
dc.date.accessioned2015-03-31T20:47:03Z
dc.date.available2015-03-31T20:47:03Z
dc.date.issued2014
dc.description.abstractThe lack of an accurate diagnosis has been a serious obstacle to the advancement of the anti-Trypanosoma cruzi chemotherapy and long-term infection can result in different health risks to human. PCRs are alternative methods, more sensitive than conventional parasitological techniques, which due to their low sensitivities are considered unsuitable for these purposes. The aim of this study was to investigate a sensitive diagnostic strategy to quantify blood and cardiac tissues parasites based on real-time PCR tools during acute and chronic phases of murine Chagas disease, as well as to monitor the evolution of infection in those mice under specific treatment. In parallel, fresh blood examination, immunological analysis and quantification of cardiac inflammation were also performed to confront and improve real-time PCR data. Similar profiles of parasitemia curves were observed in both quantification techniques during the acute phase of the infection. In contrast, parasites could be quantified only by real-time PCR at 60 and 120 days of infection. In cardiac tissue, real-time PCR detected T. cruzi DNA in 100% of infected mice, and using this tool a significant Pearson correlation between parasite load in peripheral blood and in cardiac tissue during acute and chronic phases was observed. Levels of serum CCL2, CCL5 and nitric oxide were coincident with parasite load but focal and diffuse mononuclear infiltrates was observed, even with significant (p < 0.05) reduction of parasitism after 60 days of infection. Later, this methodology was used to monitor the evolution of infection in animals treated with itraconazole (Itz). Itz-treatment induced a reduction of parasite load in both blood and cardiac muscle at the treatment period, but after the end of chemotherapy an increase of parasitism was detected. Interestingly, inflammatory mediators levels and heart inflammation intensity had similar evolution to the parasite load, in the group of animals treated. Taken together, our data show that real-time PCR strategy used was suitable for studies of murine T. cruzi infection and may prove useful in investigations involving experimental chemotherapy of the disease and the benefits of treatment in relation to parasitism and inflammatory respons.pt_BR
dc.identifier.citationGAVA, S. G. et al. Heterologous expression in Caenorhabditis elegans as an alternative approach to functional studies in Schistosoma mansoni. Frontiers in Genetics, v. 5, p. 1-5, 2014. Disponível em: <https://www.frontiersin.org/articles/10.3389/fgene.2014.00120/full>. Acesso em: 15 out. 2014.pt_BR
dc.identifier.doihttps://dx.doi.org/10.3389%2Ffgene.2014.00120
dc.identifier.issn1662-453X
dc.identifier.urihttp://www.repositorio.ufop.br/handle/123456789/4829
dc.language.isoen_USpt_BR
dc.rights.licenseThe use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Fonte: o próprio artigo.pt_BR
dc.subjectSchistosoma mansonipt_BR
dc.subjectHeterologous expressionpt_BR
dc.subjectCaenorhabditis eleganspt_BR
dc.titleHeterologous expression in Caenorhabditis elegans as an alternative approach to functional studies in Schistosoma mansoni.pt_BR
dc.typeArtigo publicado em periodicopt_BR
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