Quorum sensing regulated phenotypes in Aeromonas hydrophila ATCC 7966 deficient in AHL production.
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Date
2016
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Abstract
The virulence of Aeromonas hydrophila is positively
regulated by a quorum sensing (QS) system based on the
ahyRI genes. The aim of this study was to evaluate the phenotypic
characteristics related to virulence in the
A. hydrophila strain ATCC 7966 in order to check if virulence
traits are still functioning, despite the absence of a well characterized
QS system. As expected, we were not able to detect
quorum signaling AHL molecules in A. hydrophila ATCC
7966 by cross-streaking or thin layer chromatography with
AHL biosensor strains. Although A. hydrophila ATCC 7966
did not produce AHLs, transcription of the ahyI and ahyR
genes was observed by RT-PCR. Phenotypes generally recognized
as QS-regulated in A. hydrophila were maintained in
ATCC 7966; these phenotypes included proteolytic activity
on casein and gelatin and amylolytic, lipolytic, and β-
hemolytic activities, as well as the ability to adhere to surfaces
and to form biofilms. Moreover, the addition of exogenous
AHLs did not induce changes in the evaluated phenotypes.
The results suggest that although this strain does not produce
AHL as evaluated in this study, it maintains the phenotypes
commonly associated with QS in this species indicating that
other regulatory mechanisms independent of ahyRI may be in
place.
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Keywords
Biofilm, Hydrolytic activity
Citation
ROSSI, A. dos R. P. et al. Quorum sensing regulated phenotypes in Aeromonas hydrophila ATCC 7966 deficient in AHL production. Annals of Microbiology, v. 66, n. 3, p. 1117-11126, set. 2016. Disponível em: <https://link.springer.com/article/10.1007/s13213-016-1196-4>. Acesso em: 05 ago. 2017.